Increased frequencies of circulating CXCL10-, CXCL8- and CCL4-producing monocytes and Siglec-3-expressing myeloid dendritic cells in systemic sclerosis patients

Autor: José António Pereira da Silva, Joel A G van Roon, Tiago Carvalheiro, Hélder Trindade, Maria João Salvador, Timothy R D J Radstake, Sara Horta, Mariana Santiago, Artur Paiva
Jazyk: angličtina
Rok vydání: 2018
Předmět:
0301 basic medicine
Male
Chemokine
Myeloid
Sialic Acid Binding Ig-like Lectin 1
Pulmonary Fibrosis
Monocytes
Non-classical monocytes
Scleroderma
Systemic/metabolism
Scleroderma
Dendrites/metabolism
Cytokines/biosynthesis
0302 clinical medicine
Monocytes/metabolism
Pulmonary fibrosis
Myeloid Cells
Chemokine CCL4
Sialic Acid Binding Ig-like Lectin 1/metabolism
biology
Chemokine CXCL10/metabolism
Myeloid Cells/metabolism
Middle Aged
3. Good health
Original Research Paper
medicine.anatomical_structure
Interleukin-8/metabolism
Cytokines
Systemic sclerosis
Female
medicine.symptom
Chemokines
Interferons/metabolism
Adult
Immunology
CCL4
Inflammation
Myeloid dendritic cells
Proinflammatory cytokine
03 medical and health sciences
Pulmonary Fibrosis/metabolism
medicine
Journal Article
CXCL10
Humans
Interleukin 8
Systemic/metabolism
Aged
030203 arthritis & rheumatology
Pharmacology
Classical monocytes
Scleroderma
Systemic
business.industry
Interleukin-8
Chemokine CCL4/metabolism
Dendrites
medicine.disease
Chemokine CXCL10
Toll-Like Receptor 4
030104 developmental biology
Toll-Like Receptor 4/metabolism
biology.protein
Interferons
business
Zdroj: Inflammation Research, 67(2), 169. Birkhauser Verlag Basel
Inflammation Research
ISSN: 1023-3830
Popis: Objective To investigate the ex vivo pro-inflammatory properties of classical and non-classical monocytes as well as myeloid dendritic cells (mDCs) in systemic sclerosis (SSc) patients. Methods Spontaneous production of CXCL10, CCL4, CXCL8 and IL-6 was intracellularly evaluated in classical, non-classical monocytes and Siglec-3-expressing mDCs from peripheral blood of SSc patients and healthy controls (HC) through flow cytometry. In addition, production of these cytokines was determined upon toll-like receptor (TLR) 4 plus Interferon-γ (IFN-γ) stimulation. Results The frequency of non-classical monocytes spontaneously producing CXCL10 was increased in both limited (lcSSc) and diffuse cutaneous (dcSSC) subsets of SSc patients and CCL4 was augmented in dcSSc patients. The proportion of CCL4-producing mDCs was also elevated in dcSSc patients and the percentage of mDCS producing CXCL10 only in lcSSc patients. Upon stimulation, the frequency of non-classical monocytes expressing CXCL8 was increased in both patient groups and mDCs expressing CXCL8 only in lcSSc. Moreover, these parameters in unsupervised clustering analysis identify a subset of patients which are characterized by lung fibrosis and reduced pulmonary function. Conclusions These data point towards a role of activated non-classical monocytes and mDCs producing enhanced levels of proinflammatory cytokines in SSc, potentially contributing to lung fibrosis. Electronic supplementary material The online version of this article (10.1007/s00011-017-1106-7) contains supplementary material, which is available to authorized users.
Databáze: OpenAIRE
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