Application of Bone Marrow-Derived Macrophages Combined with Bone Mesenchymal Stem Cells in Dual-Channel Three-Dimensional Bioprinting Scaffolds for Early Immune Regulation and Osteogenic Induction in Rat Calvarial Defects

Autor:	Kaixuan Yu, Huimin Huangfu, Qiuyue Qin, Yi Zhang, Xinming Gu, Xinchan Liu, Yidi Zhang, Yanmin Zhou
Rok vydání:	2022
Předmět:	Bone Regeneration Tissue Scaffolds Alginates Macrophages Bioprinting Mesenchymal Stem Cells Cell Differentiation Biocompatible Materials Rats Osteogenesis Animals Gelatin General Materials Science Hyaluronic Acid
Zdroj:	ACS Applied Materials & Interfaces. 14:47052-47065
ISSN:	1944-8252 1944-8244
DOI:	10.1021/acsami.2c13557
Popis:	The host immune response to biomaterials is critical for determining scaffold fate and bone regeneration outcomes. Three-dimensional (3D) bioprinted scaffolds encapsulated with living cells can improve the inflammatory microenvironment and further accelerate bone repair. Here, we screened and adopted 8% methacrylamidated gelatin (GelMA)/1% methacrylamidated hyaluronic acid (HAMA) as the encapsulation system for rat bone marrow-derived macrophages (BMMs) and 3% Alginate/0.5 mg/mL graphene oxide (GO) as the encapsulation system for rat bone mesenchymal stem cells (BMSCs), thus forming a dual-channel bioprinting scaffold. The 8% GelMA/1% HAMA/3% Alginate/0.5 mg/mL GO (8/1/3/0.5) group could form a scaffold with a stable structure, good mechanical properties, and satisfied biocompatibility. When exploring the crosstalk between BMMs and BMSCs
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::db3a2ad9d8a1113c63f9b84783ff403f https://doi.org/10.1021/acsami.2c13557 Zobrazit plný text záznamu