Polymorphic Amplified Typing Sequences (PATS) Strain Typing System Accurately Discriminates a Set of Temporally and Spatially Disparate Escherichia coli O157 Isolates Associated with Human Infection
| Autor: | Robert W. Griffin, Megan Murray, Stephen B. Calderwood, Indira T. Kudva, Nimisha Kalia, Manohar John, Ralph Timperi, Sandra Smole, Jeonifer Garren |
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| Rok vydání: | 2013 |
| Předmět: |
Genetics
Gel electrophoresis animal structures Bacteria General Immunology and Microbiology animal diseases PFGE Strain typing Biology medicine.disease_cause Article PATS Microbiology PCR fluids and secretions cardiovascular system medicine Pulsed-field gel electrophoresis Strain-typing Typing O157 Escherichia coli |
| Zdroj: | The Open Microbiology Journal |
| ISSN: | 1874-2858 |
| Popis: | Polymorphic Amplified Typing Sequences (PATS) is a PCR-based Escherichia coli O157 (O157) strain typing system. Here, we show that PATS compares excellently with Pulsed-Field Gel Electrophoresis (PFGE) in that both methods cluster geographically diverse O157 isolates similarly. Comparative analysis of the results obtained in this simulated “blind” study attests to the discriminating power and applicability of PATS to epidemiological/nosocomial situations. |
| Databáze: | OpenAIRE |
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