Alterations in plasminogen activation correlate with epithelial cell dysplasia grading in colorectal adenomas
| Autor: | C. Trân-Thang, Gian Dorta, P. Chaubert, E. Saraga, P. Protiva, I. Sordat, A. L. Blum, Bernard Sordat |
|---|---|
| Rok vydání: | 1998 |
| Předmět: |
Adenoma
Male Cancer Research Epithelial dysplasia Pathology medicine.medical_specialty Tissue plasminogen activator Adenoma/enzymology Adenoma/pathology Colorectal Neoplasms/enzymology Colorectal Neoplasms/pathology Enzyme Activation Epithelial Cells/enzymology Female Fibrinolysin/metabolism Humans Middle Aged Plasminogen/metabolism Plasminogen Activator Inhibitor 1/metabolism Plasminogen Activators/metabolism Prospective Studies Tissue Plasminogen Activator/metabolism Urokinase-Type Plasminogen Activator/metabolism chemistry.chemical_compound Plasminogen Activators Plasminogen Activator Inhibitor 1 medicine Fibrinolysin Urokinase T-plasminogen activator business.industry Epithelial Cells Plasminogen medicine.disease Urokinase-Type Plasminogen Activator Oncology chemistry Dysplasia Plasminogen activator inhibitor-1 Tissue Plasminogen Activator business Colorectal Neoplasms Plasminogen activator medicine.drug Research Article |
| Zdroj: | British Journal of Cancer British journal of cancer, vol. 77, no. 2, pp. 297-304 |
| ISSN: | 0007-0920 |
| Popis: | Proteases are important for neoplastic invasion but a specific role for the plasminogen activator system in the progression of colorectal epithelial dysplasia to adenomatous lesions remains unclear. Consecutive tissue cryosections of 51 adenomas, 49 distant mucosa samples and five mucosa samples from control subjects were histopathologically analysed for dysplasia grade and tissue type, urokinase plasminogen activator levels and plasminogen activator inhibitor type 1 (PAI-1) using immunosorbent methods. Plasminogen activation and urokinase-mediated proteolytic activity levels were assessed using in situ zymography. Plasminogen activation and tissue-type activator levels were lower in adenomas than in mucosae (P < 0.001). PAI-1 concentration and urokinase levels were higher in adenomas than in mucosae (P < 0.001 and P < 0.001 respectively). In adenomas, urokinase concentration increased in parallel with PAI-1, but only the urokinase levels correlated with the dysplasia grade (P < 0.01). Thus, the alterations in plasminogen activation correlated with epithelial cell dysplasia grading. In the mucosa to adenoma transition, a marked decrease in tissue-type plasminogen activator occurred. In adenomas, this decrease was accompanied by a concomitant increase in urokinase and PAI-1. The urokinase level only continued to rise in parallel with the dysplasia grade. Resulting protease-antiprotease imbalance in high-grade dysplasia may represent the phenotypic change associated with malignant transformation and invasive behaviour. Images Figure 2 |
| Databáze: | OpenAIRE |
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