Lung carcinoma spheroids embedded in a microfluidic platform
| Autor: | Ece Yildiz-Ozturk, Ozlem Yesil-Celiktas, Pelin Saglam-Metiner |
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| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Breast-Cancer Cells Ginsenosides Cytotoxicity Proliferation Clinical Biochemistry Biomedical Engineering Transport Apoptosis Bioengineering Matrix (biology) 3-Dimensional Cell-Culture 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Lung carcinoma Panaxatriol medicine Viability assay Lung cancer 3D spheroid culture Matrigel Chemistry Spheroid Cell Biology medicine.disease Perfusion 030104 developmental biology In-Vitro Cell culture 030220 oncology & carcinogenesis embryonic structures Cancer cell Original Article Tumor-Model Microfluidic system Biotechnology Biomedical engineering |
| Zdroj: | Cytotechnology |
| ISSN: | 1573-0778 0920-9069 |
| Popis: | Three-dimensional (3D) spheroid cell cultures are excellent models used in cancer biology research and drug screening. The objective of this study was to develop a lung carcinoma spheroid based microfluidic platform with perfusion function to mimic lung cancer pathology and investigate the effect of a potential drug molecule, panaxatriol. Spheroids were successfully formed on agar microtissue molds at the end of 10 days, reaching an average diameter of about 317.18 ± 4.05 ?m and subsequently transferred to 3D dynamic microfluidic system with perfusion function. While the size of the 3D spheroids embedded in the Matrigel matrix in the platform had gradually increased both in the static and dynamic control groups, the size of the spheroids were reduced and fragmented in the drug treated groups. Cell viability results showed that panaxatriol exhibited higher cytotoxic effect on cancer cells than healthy cells and the IC50 value was determined as 61.55 µM. Furthermore, panaxatriol has been more effective on single cells around the spheroid structure, whereas less in 3D spheroid tissues with a compact structure in static conditions compared to dynamic systems, where a flow rate of 2 µL/min leading to a shear stress of 0.002 dyne/cm2 was applied. Application of such dynamic systems will contribute to advancing basic research and increasing the predictive accuracy of potential drug molecules, which may accelerate the translation of novel therapeutics to the clinic, possibly decreasing the use of animal models. Graphic abstract: [Figure not available: see fulltext.] © 2021, The Author(s), under exclusive licence to Springer Nature B.V. 113M050 Türkiye Bilimsel ve Teknolojik Araştirma Kurumu, TÜBITAK The financial support provided by the Scientific and Technological Research Council of Turkey (TUBITAK) through 113M050 Grant is highly appreciated. |
| Databáze: | OpenAIRE |
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