Species boundaries within Saprolegnia (Saprolegniales, Oomycota) based on morphological and DNA sequence data
| Autor: | David E. Padgett, J. Craig Bailey, Jonathan P. Hulvey |
|---|---|
| Rok vydání: | 2007 |
| Předmět: |
biology
Phylogenetic tree Physiology Zoology Cell Biology General Medicine Saprolegnia biology.organism_classification DNA Ribosomal DNA sequencing Phylogenetics Genus 28S ribosomal RNA DNA Ribosomal Spacer RNA Ribosomal 28S Genetics Internal transcribed spacer DNA Fungal Clade Molecular Biology Phylogeny Ecology Evolution Behavior and Systematics |
| Zdroj: | Mycologia. 99:421-429 |
| ISSN: | 1557-2536 0027-5514 |
| DOI: | 10.3852/mycologia.99.3.421 |
| Popis: | Saprolegnia is a common and widespread genus of Oomycetes, however species identifications are difficult and uncertain. To test whether keys based on morphological characters could identify species as determined by molecular characters we determined partial DNA sequences for the 28S rRNA gene and the complete internal transcribed spacer (ITS) region for 55 isolates belonging to Saprolegnia and one isolate of Protoachlya hypogyna that exhibited saprolegnoid zoospore discharge in water culture. Phylogenetic analyses of the combined sequence data yielded 10 robustly supported clades that probably represent separate species. Morphological analyses of all isolates revealed that each DNA-based clade could be delimited from others by autapomorphic or unique combinations of morphological character states but not without employing several features previously not used at the species level. Taxonomic implications of these results are discussed and recommendations for less equivocal characterization of new Saprolegnia species are made. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|