Expression of a bacterial endoglucanase gene in tobacco increases digestibility of its cell wall fibers

Autor:	Masaru Shibata, Kunio Ohmiya, Jia-Lin Sun, Tetsuya Kimura, Tetsu Kawazu, Kazuo Sakka
Rok vydání:	1999
Předmět:	biology Transgene Nicotiana tabacum fungi food and beverages Bioengineering Genetically modified crops Cellulase biology.organism_classification Applied Microbiology and Biotechnology Cell wall Biochemistry Cytoplasm Gene expression biology.protein Solanaceae Biotechnology
Zdroj:	Journal of Bioscience and Bioengineering. 88:421-425
ISSN:	1389-1723
DOI:	10.1016/s1389-1723(99)80220-5
Popis:	Transgenic tobacco plants exhibiting exogenous cellulase activity were produced by introduction of a truncated cellulase (t-EgI) gene from Ruminococcus albus . They accumulated about 0.1–0.5% t-EgI of the total soluble proteins in the cytoplasm. They were normal in morphology and growth. t-EgI expressed in the transgenic plants degraded their own cell wall materials after the cells were disrupted. The plants expressing the t-egI gene could be used as “cellulase-encapsulating plants” for livestock and food industries.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::db3369aedf4d359915b114fea5b08b5b https://doi.org/10.1016/s1389-1723(99)80220-5 Zobrazit plný text záznamu Full Text from ScienceDirect