Leishmania major: histone H1 gene expression from the sw3 locus
| Autor: | Sabina I. Belli, Andrea Formenton, Alasdair C. Ivens, Tanja Noll, Chantal Desponds, Denise Hofer, Nicolas Fasel, Raymond Jacquet |
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| Rok vydání: | 1999 |
| Předmět: |
Immunology
Blotting Western Molecular Sequence Data Restriction Mapping Locus (genetics) Polymerase Chain Reaction Histones Histone H1 Animals Genomic library Amino Acid Sequence Cloning Molecular Gene Genomic organization DNA Primers Gene Library Leishmania major Genetics biology Base Sequence Sequence Homology Amino Acid Gene Expression Regulation Developmental General Medicine Sequence Analysis DNA DNA Protozoan Molecular biology Electrophoresis Gel Pulsed-Field genomic DNA Blotting Southern Infectious Diseases Histone biology.protein Cosmid Parasitology Electrophoresis Polyacrylamide Gel Rabbits Polymorphism Restriction Fragment Length |
| Zdroj: | Experimental parasitology. 91(2) |
| ISSN: | 0014-4894 |
| Popis: | Histone H1 in the parasitic protozoan Leishmania is a developmentally regulated protein encoded by the sw3 gene. Here we report that histone H1 variants exist in different Leishmania species and strains of L. major and that they are encoded by polymorphic genes. Amplification of the sw3 gene from the genome of three strains of L. major gave rise to different products in each strain, suggesting the presence of a multicopy gene family. In L. major, these genes were all restricted to a 50-kb Bg/II fragment found on a chromosomal band of 1.3 Mb (chromosome 27). The detection of RFLPs in this locus demonstrated its heterogeneity within several species and strains of Leishmania. Two different copies of sw3 (sw3.0 and sw3.1) were identified after screening a cosmid library containing L. major strain Friedlin genomic DNA. They were identical in their 5' UTRs and open reading frames, but differed in their 3' UTRs. With respect to the originally cloned copy of sw3 from L. major strain LV39, their open reading frames lacked a repeat unit of 9 amino acids. Immunoblots of L. guyanensis parasites transfected with these cosmids revealed that both copies could give rise to the histone H1 protein. The characterization of this locus will now make possible a detailed analysis of the function of histone H1 in Leishmania, as well as permit the dissection of the molecular mechanisms governing the developmental regulation of the sw3 gene. |
| Databáze: | OpenAIRE |
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