A comparative pattern of lectin-binding in the endometrial glands of the uterus and placenta of healthy buffaloes and bovines at early gestation
| Autor: | Claudio Gustavo Barbeito, Juan Manuel Sala, L. Lischinsky, Carlos Manuel Campero, Sergio Gastón Caspe, José Luis Konrad, Dadin Prando Moore |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
Uterus
Lectinas Water Buffaloes Biology purl.org/becyt/ford/1 [https] Andrology Gestación Pregnancy Lectins WATER BUFFALO Placenta Lectin binding medicine PLACENTA purl.org/becyt/ford/1.6 [https] Early gestation GLYCOSYLATION PATTERN General Medicine Búfalo de Agua LECTIN-HISTOCHEMISTRY medicine.anatomical_structure Water buffalo Ganado Bovino Cattle purl.org/becyt/ford/4.3 [https] purl.org/becyt/ford/4 [https] Endometrial glands |
| Zdroj: | CONICET Digital (CONICET) Consejo Nacional de Investigaciones Científicas y Técnicas instacron:CONICET Biocell (Mendoza) 45 (2) : 331-337. (2021) INTA Digital (INTA) Instituto Nacional de Tecnología Agropecuaria instacron:INTA |
| ISSN: | 1667-5746 |
| Popis: | Water buffalo (Bubalus bubalis) and domestic cattle (Bos taurus) are closely related species. However, embryo transfer interspecies has been attempted without any success. The failure in hybrid embryo-implantation is associated with the glycocode in the maternal-fetal interface. Glycosylation patterns have been studied in different species of ruminants; however, in B. bubalis, only the binucleated cells (BNC) have been analyzed. This glycocode is essential for a successful embryo-implantation and can be defined by Lectin-Histochemistry (LHC). The aim of this study is to compare the glycosylation pattern of placenta and uterus in water buffaloes and cattle by LHC. Tissue samples of placenta and uterus from pregnant Mediterranean female water buffaloes (Buf1) and Angus cows (Bov1) were analyzed. All animals were euthanized at 98 days of gestation. LHC was carried out using twelve lectins (Con A, LCA, PSA, sWGA, PHA-e, SBA, UEA-1, WGA, RCA-1, PNA, DBA, BSA-1). The intensity of lectin binding was semiquantitatively scored using a scale of 0 (negative) to 3 (strongly positive). Difference between species was found in trophoblast layer by PSA, SWGA, PNA and BSA-1, in BNC, and in the mononuclear cells by LCA, PSA, PHA-e, DBA, BSA-1, PNA. In utero, differences in the apical cellular membrane and the secretion of glands were identified by DBA and RCA-1, and in the cytoplasm of those glandular epithelial cells by PHA-e, BSA-1, WGA, and SBA. In both species, BNC presented a strong positive reaction with DBA and SBA, a moderate response by LCA, PHA-e, BSA-1 and PNA lectin, and a low reaction by PSA, UEA-1, SWGA, WGA, Con A and RCA-1. The results found in this study suggest that although both species are closely related, glycosylation patterns in the placenta and uterus are different, thus providing a possible reason for embryo transfer not being possible between these species. Fil: Caspe, Sergio Gastón. Instituto Nacional de Tecnologia Agropecuaria. Centro Regional Buenos Aires Norte. Estacion Experimental Agropecuaria Pergamino. Agencia de Extension Rural Mercedes.; Argentina Fil: Konrad, José Luis. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; Argentina. Universidad Nacional del Nordeste. Facultad de Cs.veterinarias. Departamento de Producción Animal; Argentina Fil: Moore, Dadin Prando. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires Sur. Estación Experimental Agropecuaria Balcarce. Agencia de Extensión Rural Balcarce; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Sala, Juan Manuel. Instituto Nacional de Tecnologia Agropecuaria. Centro Regional Buenos Aires Norte. Estacion Experimental Agropecuaria Pergamino. Agencia de Extension Rural Mercedes.; Argentina Fil: Lischinsky, Lilian. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires Sur. Estación Experimental Agropecuaria Balcarce. Agencia de Extensión Rural Balcarce; Argentina Fil: Campero, Carlos Manuel. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires Sur. Estación Experimental Agropecuaria Balcarce. Agencia de Extensión Rural Balcarce; Argentina Fil: Barbeito, Claudio Gustavo. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Cátedra de Histología y Embriología Animal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|