Total RNA and genomic DNA of Lactobacillus gasseri OLL2809 induce interleukin-12 production in the mouse macrophage cell line J774.1 via toll-like receptors 7 and 9
Autor: | Ayako Goto, Asako Sato, Toshihiro Sashihara, Junko Mochizuki, Kazumasa Onishi |
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Rok vydání: | 2020 |
Předmět: |
DNA
Bacterial Microbiology (medical) Total RNA Phagocytosis Cell lcsh:QR1-502 Cell Culture Techniques Genomic DNA Lactobacillus gasseri Microbiology lcsh:Microbiology Cell Line Mice 03 medical and health sciences Immune system medicine Animals 030304 developmental biology 0303 health sciences Membrane Glycoproteins biology 030306 microbiology Macrophages Interleukin biology.organism_classification Interleukin-12 Lactobacillus gasseri OLL2809 Molecular biology Up-Regulation RNA Bacterial genomic DNA medicine.anatomical_structure Toll-Like Receptor 7 IL-12 Cell culture Toll-Like Receptor 9 Myeloid Differentiation Factor 88 Interleukin 12 Genome Bacterial Signal Transduction Research Article |
Zdroj: | BMC Microbiology BMC Microbiology, Vol 20, Iss 1, Pp 1-9 (2020) |
ISSN: | 1471-2180 |
Popis: | Background Lactobacillus gasseri OLL2809 can highly induce interleukin (IL)-12 production in immune cells. Even though beneficial properties of this strain for both humans and animals have been reported, the mechanism by which the bacteria induces the production of IL-12 in immune cells remains elusive. In this study, we investigated the mechanism of induction of IL-12 using a mouse macrophage cell line J774.1. Results Inhibition of phagocytosis of L. gasseri OLL2809, and myeloid differentiation factor 88 and Toll-like receptors (TLRs) 7 and 9 signalling attenuated IL-12 production in J774.1 cells. Total RNA and genomic DNA of L. gasseri OLL2809, when transferred to the J774.1 cells, also induced IL-12 production. The difference in the IL-12-inducing activity of Lactobacilli is attributed to the susceptibility to phagocytosis, but not to a difference in the total RNA and genomic DNA of each strain. Conclusion We concluded that total RNA and genomic DNA of phagocytosed L. gasseri OLL2809 induce IL-12 production in J774.1 cell via TLRs 7 and 9, and the high IL-12-inducing activity of L. gasseri OLL2809 is due to its greater susceptibility to phagocytosis. |
Databáze: | OpenAIRE |
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