The Role of Oxidative Stress and Inflammation in Conjunctivochalasis
| Autor: | Osama M.A. Ibrahim, Murat Dogru, Minako Kaido, Kazuno Negishi, Ayako Igarashi, Kazuo Tsubota, Samantha K. Ward, Yukihiro Matsumoto, Yoko Ogawa, Cristina M. Schnider, Chikako Katakami, Reiko Ishida, Tais Hitomi Wakamatsu, Jun Shimazaki |
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| Rok vydání: | 2010 |
| Předmět: |
Male
medicine.medical_specialty Pathology Conjunctiva Enzyme-Linked Immunosorbent Assay medicine.disease_cause Conjunctival Diseases Immunoenzyme Techniques Epithelial Damage chemistry.chemical_compound medicine Rose bengal Humans Prospective Studies Aged Inflammation business.industry Lysine Deoxyguanosine Conjunctivochalasis medicine.disease eye diseases Staining Oxidative Stress medicine.anatomical_structure Matrix Metalloproteinase 9 Vital stain chemistry 8-Hydroxy-2'-Deoxyguanosine Tears Cytokines Female Matrix Metalloproteinase 3 Histopathology Lipid Peroxidation business Oxidative stress DNA Damage |
| Zdroj: | Investigative Opthalmology & Visual Science. 51:1994 |
| ISSN: | 1552-5783 |
| DOI: | 10.1167/iovs.09-4130 |
| Popis: | Purpose. To investigate the status of oxidative stress and histopathologic alterations in patients with conjunctivochalasis and compare the findings with those in healthy control subjects. Methods. Eleven patients (n = 20 eyes) with Yokoi grade 3 conjunctivochalasis and 11 health control subjects (n = 22 eyes) were prospectively recruited. ELISA for tear hexanoyl-lysine (HEL) and inflammatory cytokines, tear film break-up time tests, Schirmer test measurements, and fluorescein and rose bengal vital staining were performed. Conjunctival specimens obtained during surgery for conjunctivochalasis and cataract underwent immunohistochemical staining for HEL+8-OHdG, MMP-3, and MMP-9, and positively stained cells were counted. Transmission electron microscopy was also performed, with staining for elastic fibers in the conjunctival stroma. Results. The mean tear stability and vital staining scores were significantly worse in the conjunctivochalasis patients than in the control subjects. The tear HEL and tear cytokine levels showed significantly higher values in eyes with conjunctivochalasis. IL-1beta and IL-6 levels showed a significant correlation with corneal epithelial damage. IL-1beta and TNFalpha showed a significant correlation with 8-OHdG-stained cell counts. Specimens from patients with conjunctivochalasis revealed a significantly higher number of cells positively stained for HEL, 8-OHdG, MMP-3, and MMP-9 than did specimens from age- and sex-matched control subjects. Transmission electron microscopy showed decreased intercellular cohesiveness, with the conjunctival stroma showing an accumulation of elastic fibers. Conclusions. Lipid and DNA oxidative stress were present in the conjunctiva. Increased tear inflammation seemed to coexist with loss of conjunctival epithelial cohesiveness and increased collagenolytic activity, which may explain the conjunctival laxity observed in patients with conjunctivochalasis. |
| Databáze: | OpenAIRE |
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