The swaposin-like domain of potato aspartic protease (StAsp-PSI) exerts antimicrobial activity on plant and human pathogens
| Autor: | Julieta Renee Mendieta, Mariana R. Pagano, Gustavo Raúl Daleo, Roberto A. Paggi, María Gabriela Guevara, Fernando F. Muñoz |
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| Rok vydání: | 2009 |
| Předmět: |
Phytophthora
Staphylococcus aureus Lysis Aspartic Acid Proteases Cell Membrane Permeability Physiology Cell Survival Phytophthora infestans Immunoblotting Antimicrobial proteins Biochemistry Polymerase Chain Reaction Microbiology Cell wall Cellular and Molecular Neuroscience Endocrinology Biología Celular Microbiología Anti-Infective Agents Bacillus cereus Fusarium SAPLIPs Tobacco Escherichia coli Cytotoxic T cell Humans Plant Proteins Solanum tuberosum biology food and beverages Antimicrobial biology.organism_classification Plant cell Plant specific domain Recombinant Proteins Heterologous expression Bacteria |
| Zdroj: | CIC Digital (CICBA) Comisión de Investigaciones Científicas de la Provincia de Buenos Aires instacron:CICBA |
| ISSN: | 1873-5169 |
| Popis: | Plant-specific insert domain (PSI) is a region of approximately 100 amino acid residues present in most plant aspartic protease (AP) precursors. PSI is not a true saposin domain; it is the exchange of the N- and C-terminal portions of the saposin like domain. Hence, PSI is called a swaposin domain. Here, we report the cloned, heterologous expression and purification of PSI from StAsp 1 (Solanum tuberosum aspartic protease 1), called StAsp-PSI. Results obtained here show that StAsp-PSI is able to kill spores of two potato pathogens in a dose-dependent manner without any deleterious effect on plant cells. As reported for StAPs (S. tuberosum aspartic proteases), the StAsp-PSI ability to kill microbial pathogens is dependent on the direct interaction of the protein with the microbial cell wall/or membrane, leading to increased permeability and lysis. Additionally, we demonstrated that, like proteins of the SAPLIP family, StAsp-PSI and StAPs are cytotoxic to Gram-negative and Gram-positive bacteria in a dose dependent manner. The amino acid residues conserved in SP_B (pulmonary surfactant protein B) and StAsp-PSI could explain the cytotoxic activity exerted by StAsp-PSI and StAPs against Gram-positive bacteria. These results and data previously reported suggest that the presence of the PSI domain in mature StAPs could be related to their antimicrobial activity. |
| Databáze: | OpenAIRE |
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