Regulation of spermatogenesis and reproductive capacity by Igf3 in tilapia
| Autor: | Hesheng Xiao, Christopher H.K. Cheng, Qiaoyuan Zheng, Shuangshuang Qi, Yibing Li, Deshou Wang, Minghui Li, Xingyong Liu, Shengfei Dai, Mimi Jie |
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| Rok vydání: | 2019 |
| Předmět: |
Male
endocrine system Spermatocyte Biology 03 medical and health sciences Cellular and Molecular Neuroscience Nile tilapia Meiosis Somatomedins Testis medicine Animals RNA Messenger Spermatogenesis Molecular Biology Zebrafish Cell Proliferation Pharmacology Mice Knockout 0303 health sciences Spermatid Base Sequence Reproduction 030302 biochemistry & molecular biology Wild type Gene Expression Regulation Developmental Cell Biology biology.organism_classification Sperm Spermatogonia Cell biology medicine.anatomical_structure Mutation Androgens Molecular Medicine CRISPR-Cas Systems Signal Transduction Tilapia |
| Zdroj: | Cellular and molecular life sciences : CMLS. 77(23) |
| ISSN: | 1420-9071 |
| Popis: | A novel insulin-like growth factor (igf3), which is exclusively expressed in the gonads, has been widely identified in fish species. Recent studies have indicated that Igf3 regulates spermatogonia proliferation and differentiation in zebrafish; however, detailed information on the role of this Igf needs further in vivo investigation. Here, using Nile tilapia (Oreochromis niloticus) as an animal model, we report that igf3 is required for spermatogenesis and reproduction. Knockout of igf3 by CRISPR/Cas9 severely inhibited spermatogonial proliferation and differentiation at 90 days after hatching, the time critical for meiosis initiation, and resulted in less spermatocytes in the mutants. Although spermatogenesis continued to occur later, more spermatocytes and less spermatids were observed in the igf3−/− testes when compared with wild type of testes at adults, indicating that Igf3 regulates spermatocyte to spermatid transition. Importantly, a significantly increased occurrence of apoptosis in spermatids was observed after loss of Igf3. Therefore, igf3−/− males were subfertile with drastically reduced semen volume and sperm count. Conversely, the overexpression of Igf3 in XY tilapia enhanced spermatogenesis leading to more spermatids and sperm count. Transcriptomic analysis revealed that the absence of Igf3 resulted in dysregulation of many genes involved in cell cycle, meiosis and pluripotency regulators that are critical for spermatogenesis. In addition, in vitro gonadal culture with 17α-methyltetosterone (MT) and 11-ketotestosterone (11-KT) administration and in vivo knockout of cyp11c1 demonstrated that igf3 expression is regulated by androgens, suggesting that Igf3 acts downstream of androgens in fish spermatogenesis. Notably, the igf3 knockout did not affect body growth, indicating that this Igf specifically functions in reproduction. Taken together, our data provide genetic evidence for fish igf3 in the regulation of reproductive capacity by controlling spermatogenesis. |
| Databáze: | OpenAIRE |
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