Forces driving epithelial wound healing
| Autor: | Benoit Ladoux, Xavier Trepat, Vito Conte, G. Wayne Brodland, Julien Colombelli, Mukund Gupta, Agustí Brugués, José J. Muñoz, Ester Anon, Jim H. Veldhuis |
|---|---|
| Přispěvatelé: | Institut de Bioenginyeria de Catalunya, ICREA (IBEC), Universitat de Barcelona (UB)-Facultat de Medicina, Institut Jacques Monod (IJM (UMR_7592)), Université Paris Diderot - Paris 7 (UPD7)-Centre National de la Recherche Scientifique (CNRS), Department of Civil and Environmental Engineering [Waterloo], University of Waterloo [Waterloo], Mechanobiology Institute [Singapore] (MBI), National University of Singapore (NUS), Institute for Research in Biomedicine (IRB), University of Barcelona, Laboratori de Càlcul Numèric (LACAN) (LaCàN), Universitat Politècnica de Catalunya [Barcelona] (UPC), Institució Catalana de Recerca i Estudis Avançats (ICREA), Unitat de Biofísica i Bioenginyeria [Barcelona], Universitat de Barcelona (UB)-Centro de Investigación Biomédica en Red Enfermedades Respiratorias (CIBERES)-Facultat de Medicina [Barcelona], Fondation pour la Recherche Médicale, Spanish Ministry for Economy and Competitiveness (BFU2012-38146), Juan de la Cierva Fellowship JCI-2012-15123, European Research Council (Grant Agreement 242993), Agence Nationale de la Recherche (ANR 2010 BLAN 1515), Institut Universitaire de France, Human Frontier Science Program (grant RGP0040/2012), Mechanobiology Institute of Singapore, Natural Sciences and Engineering Research Council of Canada (NSERC), Universitat Politècnica de Catalunya. Departament de Matemàtica Aplicada III, Universitat Politècnica de Catalunya. LACÀN - Mètodes Numèrics en Ciències Aplicades i Enginyeria |
| Rok vydání: | 2014 |
| Předmět: |
Engineering
Civil medicine.medical_treatment Engineering Multidisciplinary General Physics and Astronomy Nanotechnology Article Focal adhesion Actomyosin ring medicine Engineering Ocean Engineering Aerospace Engineering Biomedical ComputingMilieux_MISCELLANEOUS Actin [PHYS]Physics [physics] Physics integumentary system Enginyeria biomèdica [Àrees temàtiques de la UPC] Traction (orthopedics) Computer Science Software Engineering Engineering Marine Engineering Manufacturing Engineering Mechanical Multicellular organism Engineering Industrial Biophysics Enginyeria biomèdica Wound closure Cellular motility Wound healing Biomedical engineering |
| Zdroj: | Nature physics Nature Physics Nature Physics, Nature Publishing Group, 2014, 10 (9), pp.683-690. ⟨10.1038/nphys3040⟩ UPCommons. Portal del coneixement obert de la UPC Universitat Politècnica de Catalunya (UPC) ResearcherID Recercat. Dipósit de la Recerca de Catalunya Universitat Jaume I |
| ISSN: | 1745-2481 1745-2473 1476-4636 |
| DOI: | 10.1038/nphys3040 |
| Popis: | A fundamental feature of multicellular organisms is their ability to self-repair wounds through the movement of epithelial cells into the damaged area. This collective cellular movement is commonly attributed to a combination of cell crawling and ‘purse-string’ contraction of a supracellular actomyosin ring. Here we show by direct experimental measurement that these two mechanisms are insufficient to explain force patterns observed during wound closure. At early stages of the process, leading actin protrusions generate traction forces that point away from the wound, showing that wound closure is initially driven by cell crawling. At later stages, we observed unanticipated patterns of traction forces pointing towards the wound. Such patterns have strong force components that are both radial and tangential to the wound. We show that these force components arise from tensions transmitted by a heterogeneous actomyosin ring to the underlying substrate through focal adhesions. The structural and mechanical organization reported here provides cells with a mechanism to close the wound by cooperatively compressing the underlying substrate. |
| Databáze: | OpenAIRE |
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