High molecular weight phospholipase A2 and fatty acids in human colon tumours and associated normal tissue
| Autor: | P.K. Weech, N.M. Tremblay, A.S. Soydan, Alan Bennett, I A Tavares |
|---|---|
| Rok vydání: | 1996 |
| Předmět: |
Adult
Male Cancer Research medicine.medical_specialty Colon Immunoblotting Biology Phospholipases A chemistry.chemical_compound Phospholipase A2 Internal medicine Submucosa medicine Humans Aged Aged 80 and over chemistry.chemical_classification Arachidonic Acid Fatty Acids Fatty acid Cancer Biological activity Middle Aged medicine.disease Molecular Weight Phospholipases A2 Enzyme Endocrinology medicine.anatomical_structure Oncology chemistry Cytoplasm Colonic Neoplasms biology.protein Female Arachidonic acid |
| Zdroj: | European Journal of Cancer. 32:1781-1787 |
| ISSN: | 0959-8049 |
| DOI: | 10.1016/0959-8049(96)00166-9 |
| Popis: | Human colon tumours usually form more prostaglandins (PGs) than associated normal tissues, but the mechanism(s) are not fully understood. We analysed fatty acid compositions, in particular arachidonate, and measured the amount and the activity of high molecular weight cytoplasmic phospholipase A2 (cPLA2) of these tissues. Total lipids extracted from homogenised surgical specimens were transesterified and fatty acids were analysed by gas chromatography. cPLA2 was separated by SDS-PAGE, Western-blotted, immunoblotted using a specific antibody to cPLA2 and semiquantified following enhanced chemiluminescence using a scanning densitometer. cPLA2 biological activity was also assayed using 1-stearoyl, 2-[1-14C]-arachidonyl, L-3-phosphatidylcholine. Compared with normal mucosa/submucosa, there was more total arachidonate in tumours (P < 0.01), and increased levels of cPLA2 occurred in 6 of 17 tumours. In conclusion, the higher amounts of tumour total arachidonate and the sometimes higher levels of cPLA2, might help to explain why some human colon tumours form increased amounts of PGs. |
| Databáze: | OpenAIRE |
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