Urease-Positive Bacteria Other thanHelicobacter pyloriin Human Gastric Juice and Mucosa
| Autor: | Marta Granata, Guido Biasco, Bruno Biavati, Paola Mattarelli, Giovanni Brandi, Carlo Calabrese, Gioconda Saccoccio, Giulio Di Febo, Anna Nannetti |
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| Přispěvatelé: | Brandi G., Biavati B., Calabrese C., Granata M., Nannetti A., Mattarelli P., Di Febo G., Saccoccio G., Biasco G. |
| Rok vydání: | 2006 |
| Předmět: |
Adult
Male medicine.medical_specialty Urease Biopsy Urea breath test Spirillaceae Rapid urease test Gastroenterology Microbiology Internal medicine Gastroscopy medicine Humans Antrum Aged Gastric Juice Bacteria Hepatology medicine.diagnostic_test biology HELICOBACTER PYLORI Hydrogen-Ion Concentration Middle Aged Helicobacter pylori HUMAN GASTRIC JUICE biology.organism_classification Staphylococcus capitis Gastric Mucosa Microscopy Electron Scanning biology.protein Female HUMAN GASTRIC MUCOSA UREASE-POSITIVE BACTERIA |
| Zdroj: | The American Journal of Gastroenterology. 101:1756-1761 |
| ISSN: | 1572-0241 0002-9270 |
| DOI: | 10.1111/j.1572-0241.2006.00698.x |
| Popis: | BACKGROUND Many bacteria carry the urease enzyme in different human ecosystems, but Helicobacter pylori is AND AIM: the only known bacterium showing urease activity in gastric ecosystems. For this reason, the rapid urease test (RUT) on gastric biopsies and urea breath test (C-UBT) are used to detect H. pylori infection.The aim of this study was to evaluate the presence of urease-positive bacteria other than H. pylori in gastric juice and mucosa in hypochlorhydric subjects. METHODS: Twenty-five hypochlorhydric and 10 normochlorhydric patients were analyzed for the presence of H. pylori and bacterial overgrowth both in gastric juice and on the mucosa. During upper gastrointestinal endoscopy at 8.00 a.m. gastric juice samples and biopsy specimens were taken from the antrum and corpus. All samples were analyzed using standard microbiological procedures like aerobic/anaerobic growth, gram-staining, gas chromatography, API test, 96-clone method, and selective medium to search for specific bacteria. In addition, all strains isolated were screened for urease activity using the CP-test. Urease positive strains were tested for the capacity to survive in an acid environment with or without urea (10 mM/L), at pH 7, 4, 3, and 2, respectively, at different times (0, 20, 30, and 60 min). RESULTS: Six hypochlorhydric patients had 10 strains of urease-positive non-H. pylori bacteria among which Staphylococcus capitis urealiticum showed the strongest urease activity. CONCLUSIONS: Hypochlorhydric patients present many urease-positive bacteria other than H. pylori. The strong urease activity may be responsible for false positive results at RUT or UBT test in patients with suspected H. pylori infection. |
| Databáze: | OpenAIRE |
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