Cytotoxicity and estrogenicity in simulated dental wastewater after grinding of resin-based materials
| Autor: | N. Wingert, Richard Gminski, Elmar Hellwig, C. Reidelbach, Olga Polydorou, Manuel Garcia-Käufer, Kirstin Vach, Ali Arif |
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| Rok vydání: | 2021 |
| Předmět: |
Bisphenol A
Materials science Chromatography Elution Fraction (chemistry) Wastewater Composite Resins UDMA Grinding Dental Materials chemistry.chemical_compound chemistry Distilled water Mechanics of Materials Materials Testing Humans Methacrylates General Materials Science Cytotoxicity General Dentistry |
| Zdroj: | Dental Materials. 37:1486-1497 |
| ISSN: | 0109-5641 |
| Popis: | This study evaluated the cytotoxic and estrogenic effects of dust and eluates released into simulated wastewater after grinding of dental resin-based materials.Four materials were used: ceram.x® universal, Filtek™ Supreme XTE, Lava™ Ultimate and Core-X™ flow. From each composite material, samples (5 × 2 mm, n = 50) were prepared according to the manufacturers' instructions. Lava™ Ultimate was used as blocks. All samples were ground to dust with a diamond bur (106 μm) and suspended in distilled water at 60 mg/mL. After storage for 72 h, the suspensions were separated into a soluble (eluate) and a particulate (dust) fraction. Eluates and dusts were evaluated for inhibition of Vibrio fischeri bioluminescence and cytotoxicity on human A549 lung cells (WST-1-Assay). The estrogenic activity was assessed by YES-Assay using Saccharomyces cerevisiae. Additionally, dental monomers (BisGMA, BisEMA, UDMA, TEGDMA, HEMA) and Bisphenol A were investigated.All eluates showed inhibition of V. fischeri bioluminescence at concentrations above 1.1 mg/mL (p0.05). The activity of the eluates of ceram.x® universal and Filtek™ Supreme XTE was significantly higher than Lava™ Ultimate and Core-X™ flow (p0.05). In the WST-1-Assay, all materials induced cytotoxic effects at concentrations of 0.1 mg/mL (p0.05), while no significant differences were detected among them. The tested materials revealed no estrogenic activity. All dental monomers and Bisphenol A showed concentration dependent cytotoxic effects (p0.05), whereas only Bisphenol A induced an estrogenic effect (p0.01).Dust and eluates of resin-based dental materials released into wastewater exert bactericidal and cytotoxic effects in vitro. However, they reveal no estrogenic effect. |
| Databáze: | OpenAIRE |
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