Denitrifying anaerobic methane oxidation process responses to the addition of growth factor betaine in the MFC-granular sludge coupling system: Enhancing mechanism and metagenomic analysis
| Autor: | Xin Guan, Menglin Chen, Chengyuan Su, Zun Huang, Qiujin Deng, Xinya Lu, Zhengpeng Chen |
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| Rok vydání: | 2021 |
| Předmět: |
Environmental Engineering
Denitrification Nitrogen Health Toxicology and Mutagenesis 0211 other engineering and technologies 02 engineering and technology Methanothrix 010501 environmental sciences 01 natural sciences Formaldehyde assimilation chemistry.chemical_compound Denitrifying bacteria Bioreactors Betaine Ammonium Compounds Environmental Chemistry Anaerobiosis Food science Waste Management and Disposal 0105 earth and related environmental sciences 021110 strategic defence & security studies Sewage biology biology.organism_classification Pollution chemistry Anaerobic oxidation of methane Intercellular Signaling Peptides and Proteins Metagenomics Proteobacteria Euryarchaeota Methane Oxidation-Reduction |
| Zdroj: | Journal of Hazardous Materials. 416:126139 |
| ISSN: | 0304-3894 |
| DOI: | 10.1016/j.jhazmat.2021.126139 |
| Popis: | To solve the problem of the slow growth of denitrifying anaerobic methane oxidation (DAMO) bacteria during the enrichment process, betaine was added as a growth factor and its influence on the mechanism of DAMO process along with the metagenomic analysis of the process in a MFC-granular sludge coupling system was explored. When the addition of betaine was increased to 0.5 g/L and 1.0 g/L, the NO3−-N removal increased to 210 mg/L. Also, the increasing betaine dosage in 1st to 4th chambers resulted in a significant increase in dissolved methane concentration which reached a maximum value of 16.6 ± 1.19 mg/L. When the dosage of betaine was increased from 0 g/L to 1.0 g/L, the dominant bacterial phyla in the 1st to 4th chambers changed to Proteobacteria (20.8–50.7%) from Euryarchaeota (42.0–54.1%) and Methanothrix which was significantly decreased by 17.9–37.4%. There was a slight decline in the DAMO microorganism abundance, possibly due to the increased methyl donors limiting the DAMO microorganism growth. Denitrification metabolism pathway module (increased from 0.10% to 0.15%) of Nitrogen metabolism and Formaldehyde assimilation, and serine pathway of Methane metabolism presented an ascendant trend with the increased betaine dosage as determined by the metagenomics analysis of KEGG metabolism pathway. |
| Databáze: | OpenAIRE |
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