| Autor: |
Yusaku Katada, Hiromitsu Kunimi, Naho Serizawa, Deokho Lee, Kenta Kobayashi, Kazuno Negishi, Hideyuki Okano, Kenji F. Tanaka, Kazuo Tsubota, Toshihide Kurihara |
| Rok vydání: |
2023 |
| Předmět: |
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| Zdroj: |
Molecular Therapy - Methods & Clinical Development. |
| ISSN: |
2329-0501 |
| Popis: |
Ectopic induction of optogenetic actuators, such as channelrhodopsin, is a promising approach to restoring vision in the degenerating retina. However, the cell type-specific response of ectopic photoreception has not been well understood. It is limited to obtain efficient gene expression in a specifically targeted cell population by a transgenic approach. In the present study, we established a murine model with high efficiency of gene induction to retinal ganglion cells (RGCs)- and amacrine cells using an improved tetracycline transactivator-operator bipartite system (KENGE-tet system). To investigate the cell type-specific visual restorative effect, we expressed the channelrhodopsin gene into RGCs and amacrine cells using the KENGE-tet system. As a result, enhancement in the visual restorative effect was observed to RGCs and starburst amacrine cells. In conclusion, a photoresponse from amacrine cells may enhance the maintained response of RGCs and further increase/improve the visual restorative effect. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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