Antifungal activity and mechanism of action of Ou-gon (Scutellaria root extract) components against pathogenic fungi
Autor: | Osamu Yamamoto, Yayoi Nishiyama, Eishin Morita, Duerna Tie, Kyaw Zaw Hein, Xia Da |
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Jazyk: | angličtina |
Rok vydání: | 2019 |
Předmět: |
0301 basic medicine
Spectrometry Mass Electrospray Ionization Antifungal Agents Scutellaria lcsh:Medicine Trichophyton rubrum Plant Roots Article Aspergillus fumigatus 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Wogonin Trichophyton Candida albicans Mode of action lcsh:Science Membrane Potential Mitochondrial Multidisciplinary biology Plant Extracts lcsh:R Fungi biology.organism_classification Baicalein 030104 developmental biology chemistry Biochemistry Flavanones Scutellaria baicalensis lcsh:Q Reactive Oxygen Species 030217 neurology & neurosurgery Chromatography Liquid |
Zdroj: | Scientific Reports, Vol 9, Iss 1, Pp 1-12 (2019) Scientific Reports |
ISSN: | 2045-2322 |
DOI: | 10.1038/s41598-019-38916-w |
Popis: | Ou-gon, an extract from Scutellaria baicalensis Georgi root, has been shown to exhibit pronounced antifungal activity. The present study aimed to identify antifungal components of Ou-gon and to determine their mechanism of action against pathogenic fungi. Antifungal activity was assessed by the microbroth dilution method using four common human pathogenic fungi, Trichophyton rubrum, Trichophyton mentagrophytes, Aspergillus fumigatus, and Candida albicans. Components of crude Ou-gon extract were separated by reversed-phase high-performance liquid chromatography. Active antifungal components were identified by liquid chromatography-electrospray ionization tandem mass spectrometry. Terminal deoxynucleotidyl transferase dUTP nick end-labelling assay, SYTOX® green uptake assay, determination of intracellular reactive oxygen species and mitochondrial membrane potential as well as microscopy (confocal laser microscopy, scanning and transmission electron microscopy) were used to probe the mode of action. Two components with potent antifungal activity, baicalein and wogonin, were identified in Ou-gon. Baicalein showed potent antifungal activity against the four fungi tested. Wogonin displayed antifungal activity against all four fungi except C. albicans. The components are considered to induce apoptosis-like programmed cell death via hyperproduction of reactive oxygen species. This study enhances our understanding of the antifungal activity of Kampo medicine, and may contribute to the development of new and safe antifungal therapeutics. |
Databáze: | OpenAIRE |
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