Silver nanoparticles-composing alginate/gelatine hydrogel improves wound healing in vivo
| Autor: | Rubson Pinheiro Maia, L. P. da Costa, Elena Sánchez-López, L. Rannier, Luciana Nalone Andrade, Marco Vinícius Chaud, Eliana B. Souto, Patrícia Severino, Flavia Rezende Diniz, R. L. C. de Albuquerque Junior, Shabir Hassan, C. F. da Silva, Cristiane Bani Corrêa, Su Ryon Shin |
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| Přispěvatelé: | Universidade do Minho |
| Rok vydání: | 2020 |
| Předmět: |
silver nanoparticles
Biopolímers food.ingredient Ferides i lesions General Chemical Engineering Cicatrització Wound healing 02 engineering and technology 010402 general chemistry 01 natural sciences Gelatin Article Silver nanoparticle sodium alginate lcsh:Chemistry gelatin food Biopolymers In vivo medicine General Materials Science Fibroblast chemistry.chemical_classification antimicrobial activity Science & Technology Wounds and injuries Granulation tissue Polymer 021001 nanoscience & nanotechnology healing 0104 chemical sciences 3. Good health medicine.anatomical_structure lcsh:QD1-999 chemistry Self-healing hydrogels 0210 nano-technology Nuclear chemistry |
| Zdroj: | Dipòsit Digital de la UB Universidad de Barcelona Nanomaterials; Volume 10; Issue 2; Pages: 390 Repositório Científico de Acesso Aberto de Portugal Repositório Científico de Acesso Aberto de Portugal (RCAAP) instacron:RCAAP Nanomaterials Nanomaterials, Vol 10, Iss 2, p 390 (2020) |
| Popis: | Polymer hydrogels have been suggested as dressing materials for the treatment of cutaneous wounds and tissue revitalization. In this work, we report the development of a hydrogel composed of natural polymers (sodium alginate and gelatin) and silver nanoparticles (AgNPs) with recognized antimicrobial activity for healing cutaneous lesions. For the development of the hydrogel, different ratios of sodium alginate and gelatin have been tested, while different concentrations of AgNO3 precursor (1.0, 2.0, and 4.0 mM) were assayed for the production of AgNPs. The obtained AgNPs exhibited a characteristic peak between 430450 nm in the ultraviolet-visible (UVVis) spectrum suggesting a spheroidal form, which was confirmed by Transmission Electron Microscopy (TEM). Fourier Transform Infra-red (FTIR) analysis suggested the formation of strong intermolecular interactions as hydrogen bonds and electrostatic attractions between polymers, showing bands at 2920, 2852, 1500, and 1640 cm1. Significant bactericidal activity was observed for the hydrogel, with a Minimum Inhibitory Concentration (MIC) of 0.50 µg/mL against Pseudomonas aeruginosa and 53.0 µg/mL against Staphylococcus aureus. AgNPs were shown to be non-cytotoxic against fibroblast cells. The in vivo studies in female Wister rats confirmed the capacity of the AgNP-loaded hydrogels to reduce the wound size compared to uncoated injuries promoting histological changes in the healing tissue over the time course of wound healing, as in earlier development and maturation of granulation tissue. The developed hydrogel with AgNPs has healing potential for clinical applications. This research received funding from the Coordenação Aperfeiçoamento de Pessoal de Nivel Superior (CAPES), Fundação de Amparo à Pesquisa do Estado de Sergipe (FAPITEC), Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq, #443238/2014-6, #470388/2014-5), and from the Portuguese Science and Technology Foundation (FCT) projects M-ERA-NET/0004/2015 (PAIRED) and UIDB/04469/2020 (strategic fund). info:eu-repo/semantics/publishedVersion |
| Databáze: | OpenAIRE |
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