Repression activity of Tailless on h 1 and eve 1 pair-rule stripes
| Autor: | Luciano Antonio Digiampietri, Luiz Paulo Andrioli, Wesley Silva dos Santos, Francisco dos Santos Aguiar |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Embryology animal structures BIOLOGIA DO DESENVOLVIMENTO Mutant Embryonic Development Repressor Biology Interference (genetic) 03 medical and health sciences Basic Helix-Loop-Helix Transcription Factors Transcriptional regulation Animals Drosophila Proteins Blastoderm Gene Silencing Psychological repression Gene Gap gene Homeodomain Proteins Genetics fungi Gene Expression Regulation Developmental food and beverages Repressor Proteins Drosophila melanogaster 030104 developmental biology embryonic structures Transcription Factors Developmental Biology |
| Zdroj: | Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual) Universidade de São Paulo (USP) instacron:USP |
| ISSN: | 0925-4773 |
| DOI: | 10.1016/j.mod.2016.10.002 |
| Popis: | We investigated the hypothesis that several transcriptional repressors are necessary to set the boundaries of anterior pair-rule stripes in Drosophila. Specifically, we tested whether Tailless (Tll) is part of a repression mechanism that correctly sets the anterior boundaries of hairy 1 (h 1) and even-skipped 1 (eve 1) stripes. Single mutant tll embryos displayed subtle deviations from the normal positions of h 1 and eve 1 stripes. Moreover, we observed stronger stripe deviations in embryos lacking both Tll and Sloppy-paired 1 (Slp 1), a common repressor for anterior pair-rule stripes. Using h 1 and eve 1 reporter constructs in the genetic assays, we provided further evidence that interference with normal mechanisms of stripe expression is mediated by Tll repression. Indeed, Tll represses both h 1 and eve 1 reporter stripes when misexpressed. Investigating the expression of other anterior gap genes in different genetic backgrounds and in the misexpression assays strengthened Tll direct repression in the regulation of h 1 and eve 1. Our results are consistent with tll being a newly-identified component of a combinatorial network of repressor genes that control pair-rule stripe formation in the anterior blastoderm of Drosophila. |
| Databáze: | OpenAIRE |
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