Improving Cell Viability and Velocity in μ-Extrusion Bioprinting with a Novel Pre-Incubator Bioprinter and a Standard FDM 3D Printing Nozzle
| Autor: | José B. Pagador, María Duarte-León, Juan Carlos Gómez-Blanco, Manuel Matamoros, Federica Marinaro, A. Marcos, David Patrocinio, Victor Galván-Chacón, Álvaro J. Sánchez-Ortega, Francisco M. Sánchez-Margallo |
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| Rok vydání: | 2021 |
| Předmět: |
Technology
fluid flow Accuracy and precision Materials science 0206 medical engineering Plastics extrusion Nozzle 3D printing conical tip 02 engineering and technology pre-incubator Article 3d printer General Materials Science atmospheric enclosure Viability assay cell viability Microscopy QC120-168.85 nozzle business.industry QH201-278.5 Engineering (General). Civil engineering (General) 021001 nanoscience & nanotechnology 020601 biomedical engineering TK1-9971 Volumetric flow rate Descriptive and experimental mechanics Extrusion Electrical engineering. Electronics. Nuclear engineering TA1-2040 0210 nano-technology business bioprinting Biomedical engineering |
| Zdroj: | Materials Volume 14 Issue 11 Materials, Vol 14, Iss 3100, p 3100 (2021) |
| ISSN: | 1996-1944 |
| Popis: | Bioprinting is a promising emerging technology. It has been widely studied by the scientific community for the possibility to create transplantable artificial tissues, with minimal risk to the patient. Although the biomaterials and cells to be used are being carefully studied, there is still a long way to go before a bioprinter can easily and quickly produce printings without harmful effects on the cells. In this sense, we have developed a new μ-extrusion bioprinter formed by an Atom Proton 3D printer, an atmospheric enclosure and a new extrusion-head capable to increment usual printing velocity. Hence, this work has two main objectives. First, to experimentally study the accuracy and precision. Secondly, to study the influence of flow rates on cellular viability using this novel μ-extrusion bioprinter in combination with a standard FDM 3D printing nozzle. Our results show an X, Y and Z axis movement accuracy under 17 μm with a precision around 12 μm while the extruder values are under 5 and 7 μm, respectively. Additionally, the cell viability obtained from different volumetric flow tests varies from 70 to 90%. So, the proposed bioprinter and nozzle can control the atmospheric conditions and increase the volumetric flow speeding up the bioprinting process without compromising the cell viability. |
| Databáze: | OpenAIRE |
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