Enzymatic Studies of Liver Cells Obtained by Fine Needle Biopsy from Mice with the Obese-Hyperglycemic Syndrome (Genesymbol ob)
| Autor: | C. Berne, Sighild Westman-Naeser |
|---|---|
| Rok vydání: | 1978 |
| Předmět: |
Male
medicine.medical_specialty Phosphofructokinase-1 Endocrinology Diabetes and Metabolism Clinical Biochemistry Mice Obese Dehydrogenase Biology Biochemistry Mice Endocrinology Internal medicine medicine Animals Glycolysis chemistry.chemical_classification Biopsy Needle Biochemistry (medical) 3-Hydroxyacyl CoA Dehydrogenases Fatty acid General Medicine Metabolism Carbohydrate Enzyme assay Freeze Drying Enzyme Liver chemistry Hyperglycemia biology.protein Phosphofructokinase |
| Zdroj: | Hormone and Metabolic Research. 10:124-127 |
| ISSN: | 1439-4286 0018-5043 |
| DOI: | 10.1055/s-0028-1093457 |
| Popis: | In an enzymatic study of livers from obese hyperglycemic mice (obob) and their lean litter mates, microdissected freeze-dried fine needle biopsies were used. Carbohydrate, fatty acid and ketone body metabolism was examined through assays of phosphofructokinase (PFK), 3-hydroxyacyl-CoA dehydrogenase (HOADH) and D-3-hydroxybutyrate dehydrogenase (HBDH). At 2 months of age the obob-mice showed a significant increase in PFK activity as compared to their lean litter mates. No such difference was found for the activity of HOADH and HBDH. An early increase in the initial glycolysis in the livers of obob-mice might thus be one of the factors responsible for the accelerated synthesis of lipids resulting in the fatty livers of the adult obob-mice. The present results corroborate previous studies indicating no major derangement in the fatty acid and ketone body metabolism in the livers of obob-mice. In a methodological evaluation of the technique for tissue sampling and preparation, freeze-dried fine-needle punctates showed significantly higher enzyme activities of PFK and HOADH than freeze-dried liver sections or crude homogenates. Freeze-drying of fine needle biopsies appears to be a technique with good preservation of the enzyme activity. This is of significance for future metabolic studies also in humans. |
| Databáze: | OpenAIRE |
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