LC-PDA-MS/MS-Based Characterization of Key Phytochemicals in Eurycoma Longifolia Roots
| Autor: | Hoi Jin Wong, Lee Suan Chua, Abirame Segaran |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Phytochemicals
Ethyl acetate 01 natural sciences Plant Roots Analytical Chemistry chemistry.chemical_compound Column chromatography Glucoside Tandem Mass Spectrometry Eurycoma Chromatography High Pressure Liquid chemistry.chemical_classification Chromatography biology 010405 organic chemistry Plant Extracts Glycoside General Medicine biology.organism_classification Coumarin 0104 chemical sciences 010404 medicinal & biomolecular chemistry chemistry Chromone Quassinoid Eurycoma longifolia |
| Zdroj: | Journal of chromatographic science. 59(7) |
| ISSN: | 1945-239X |
| Popis: | The objective of the study was to fractionate the crude extract of Eurycoma longifolia (E. longifolia) roots and identify the intense peaks using HPLC-PDA-MS/MS, UPLC–MS/MS and H-NMR. Column chromatography was used to fractionate the crude extract into individual fractions using six solvent systems ranged from ethyl acetate, methanol and water in increasing polarity. Two fractions with nearly pure and intense peaks were selected for compound identification. Chromenone (coumarin) and chromone derivatives were putatively identified, besides several previously reported quassinoid glycosides (eurycomanone derived glycoside, 2,3-dehydro-4α-hydroxylongilactone glucoside, eurycomanol glycoside and eurycomanol trimer) in the fraction 11 of 100% methanol. A newly reported compound, namely hydroxyl glyyunanprosapogenin D (838 g/mol) was proposed to be the compound detected in the fraction 11 of 50% ethyl acetate and 50% methanol. This is also the first study to report the identification of chromenones and chromones in E. longifolia extract. |
| Databáze: | OpenAIRE |
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