Naturally Acquired IgM Antibody Response to the C-Terminal Region of the Merozoite Surface Protein 1 of Plasmodium vivax in Korea: Use for Serodiagnosis of Vivax Malaria
| Autor: | Yun Jae Jung, Seung Bum Yoo, Joon Sup Yeom, Jae-Won Park, Mi Jin Sohn, Jae Hoon Oh, Ineui Bae, Eu Suk Kim, Eun Kyung Kim, Kook Jin Lim |
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| Rok vydání: | 2008 |
| Předmět: |
Plasmodium vivax
Antibodies Protozoan Enzyme-Linked Immunosorbent Assay Sensitivity and Specificity Immune system Recurrence parasitic diseases Malaria Vivax medicine Animals Humans Mass Screening Merozoite Surface Protein 1 Ecology Evolution Behavior and Systematics Mass screening Korea biology biology.organism_classification medicine.disease Virology Immunoglobulin M Case-Control Studies Immunology Vivax malaria biology.protein Parasitology Antibody Malaria Follow-Up Studies |
| Zdroj: | Journal of Parasitology. 94:1410-1414 |
| ISSN: | 1937-2345 0022-3395 |
| DOI: | 10.1645/ge-1484.1 |
| Popis: | The antibody levels against the C-terminal region of the merozoite surface protein 1 of Plasmodium vivax (PvMSP1c) were measured in 276 patients with P. vivax malaria (patient group), 320 malaria-naïve healthy individuals (control group 1), and 70 malaria-naïve individuals with various disorders (control group 2) using the immunoglobulin M (IgM) capture enzyme-linked immunosorbent assay (ELISA) and the direct sandwich ELISA. To evaluate the antibody response during relapse, 5 relapsed patients were tested using the IgM capture ELISA. The IgM antibodies were negative in 99.7% of control group 1 and in 100% of control group 2; they were positive in 90.6% of the patient group. The total antibody levels were positive in 88.4% of the patient group with the direct sandwich ELISA. The sera from the second malaria episode, i.e., relapsed patients, were 100% positive on the IgM capture ELISA. The results of this study suggest that the IgM capture ELISA may be a useful diagnostic method for P. vivax malaria for both primary infection and relapse. |
| Databáze: | OpenAIRE |
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