Rapid analysis of diclofenac and some of its transformation products in the three-spined stickleback, Gasterosteus aculeatus, by liquid chromatography-tandem mass spectrometry

Autor: Maéva Fieu, Jean-Marc Porcher, Cyril Turies, Sandrine Joachim, Gaëlle Daniele, Anne Bado-Nilles, Emmanuelle Vulliet, Sandrine Andres, Patrick Baudoin
Přispěvatelé: TRACES - Technologie et Recherche en Analyse Chimique pour l'Environnement et la Santé, Institut des Sciences Analytiques (ISA), Institut de Chimie du CNRS (INC)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS), Institut National de l'Environnement Industriel et des Risques (INERIS), This study has received financial support from the ANSM (Agence Nationale de Securite du Medicament et des produits de sante).
Rok vydání: 2016
Předmět:
ENVIRONMENTAL RISK
QuEChERS
Diclofenac
WATER TREATMENT PLANTS
Metabolite
010501 environmental sciences
Quechers
01 natural sciences
Biochemistry
ENDOCRINE DISRUPTORS
Analytical Chemistry
DRUG DICLOFENAC
PERSONAL CARE PRODUCTS
Tandem Mass Spectrometry
SAFE EXTRACTION
[CHIM.ANAL]Chemical Sciences/Analytical chemistry
Liquid chromatography–mass spectrometry
Animals
14. Life underwater
LC-MS/MS
SURFACE WATERS
0105 earth and related environmental sciences
Abiotic component
Chromatography
Molecular Structure
biology
Chemistry
Aquatic ecosystem
010401 analytical chemistry
Extraction (chemistry)
Stickleback
BENTHIC INVERTEBRATES
Contamination
biology.organism_classification
6. Clean water
Perciformes
Photoproduct
0104 chemical sciences
13. Climate action
Bioaccumulation
Environmental chemistry
MULTICLASS PHARMACEUTICALS
Pharmaceuticals
RESIDUE ANALYSIS
Water Pollutants
Chemical
Chromatography
Liquid
Zdroj: Analytical and Bioanalytical Chemistry
Analytical and Bioanalytical Chemistry, Springer Verlag, 2016, 408 (16), pp.4435-4444. ⟨10.1007/s00216-016-9541-9⟩
ISSN: 1618-2650
1618-2642
DOI: 10.1007/s00216-016-9541-9
Popis: Pharmaceuticals are emerging organic contaminants ubiquitously present in the environment due to incessant input into the aquatic compartment mainly resulting from incomplete removal in wastewater treatment plants. One of the major preoccupations concerning pharmaceuticals released into surface waters is their potential for bioaccumulation in biota, possibly leading to deleterious effects on ecosystems especially as they could affect a broad variety of organisms living in or depending on the aquatic environment. Thus, the development of accurate and sensitive methods is necessary to detect these compounds in aquatic ecosystems. Considering this need, this study deals with the analytical development of a methodology to quantify traces of diclofenac together with some of its biotic and abiotic transformation products in whole-body tissue of three-spined stickleback. A simple and reliable extraction method based on a modified QuEChERS extraction is implemented on 200 mg of fish. The detection and quantification of the ten target compounds are performed using liquid chromatography-tandem mass spectrometry. The whole process was successfully validated regarding linearity, recovery, repeatability, and reproducibility. The method limits of detection and quantification do not exceed 1 ng/g. To reproduce environmental conditions, we measured the concentration of DCF and its transformation products in three-spined sticklebacks after a 6-month exposure in mesocosms at several levels of DCF ranging from 0.05 to 4.1 μg/L. The phase I metabolite 4'-hydroxydiclofenac was detected in fish samples exposed at the highest DCF concentration. Graphical abstract Analysis of diclofenac and some of its transformation products in the three-spined stickleback, Gasterosteus aculeatus, by QuEChERS extraction followed by LC-MS/MS.
Databáze: OpenAIRE
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