Spatiotemporal PFQNM visualization of the effect of suicide dendriplexes on dividing HeLa cells
| Autor: | Matthew Jay Quast, Serag Eldin I. Elbehairi, Anja Mueller, Hosam G. Abdelhady, Esmaeel M. Niazy, Hassan A. Abdel-Salam, Ahmed M. Effat |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Materials science Cell division Cell Biomedical Engineering Pharmaceutical Science Medicine (miscellaneous) Bioengineering 02 engineering and technology Microscopy Atomic Force Thymidine Kinase HeLa Cell membrane 03 medical and health sciences Elastic Modulus medicine Humans Simplexvirus General Materials Science Cytoskeleton biology Cell Membrane Genes Transgenic Suicide Suicide gene 021001 nanoscience & nanotechnology biology.organism_classification Virology Cell biology 030104 developmental biology medicine.anatomical_structure Apoptosis Cancer cell Molecular Medicine 0210 nano-technology HeLa Cells |
| Zdroj: | Nanomedicine : nanotechnology, biology, and medicine. 12(8) |
| ISSN: | 1549-9642 |
| Popis: | Suicide gene delivery is significant in cancer therapy but has not been fully investigated on a cellular scale. Here, Peak Force Quantitative Nanomechanical atomic force microscopy (PFQNM-AFM) was applied to visualize the effect of herpes simplex virus thymidine kinase dendriplexes (G4AcFaHSTK) on the morphological and nanomechanical properties of individual live and dividing HeLa cells. Cells were then exposed to G4AcFaHSTK, followed by ganciclovir, and directly imaged by real-time PFQNM-AFM. Cell membrane liquefaction, cytoplasmic shrinkage, and cytoskeleton structure loss were observed during cell division. The average Young's modulus of the nuclear region increased with time as the cell continued from metaphase (6.29 kPa) to telophase (13.6 kPa) and then decreased (2.25 kPa) upon apoptosis. In contrast, cells exposed to either ganciclovir or G4AcFaHSTK alone have no changes. Thus, understanding the real-time effects of suicide dendriplexes on the cytoskeletal and nanomechanical behaviors of cancer cells may provide new methods for cancer treatment. |
| Databáze: | OpenAIRE |
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