Utilization of sucrose during cocultivation positively affects Agrobacterium-mediated transformation efficiency in sugar beet (Beta vulgaris L.)
| Autor: | Ali Ergül, Canan Yüksel Özmen, Muhammet Çağri Oğuz, Kemal Kazan, Ekrem Gürel, Ferzat Turan, Songül Gürel |
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| Přispěvatelé: | BAİBÜ, Fen Edebiyat Fakültesi, Biyoloji Bölümü, Gürel, Songül, Gürel, Ekrem |
| Rok vydání: | 2019 |
| Předmět: |
0106 biological sciences
Sucrose Agrobacterium 01 natural sciences 040501 horticulture chemistry.chemical_compound Murashige and Skoog medium Sugar beet genetic transformation cocultivation plant growth regulators Plant Growth Regulators Ecology biology Chemistry fungi food and beverages Genetic Transformation Cocultivation Forestry 04 agricultural and veterinary sciences Agrobacterium tumefaciens biology.organism_classification Transformation (genetics) Horticulture Sugar Beet Sugar beet 0405 other agricultural sciences 010606 plant biology & botany Food Science Explant culture Transformation efficiency |
| Zdroj: | Volume: 43, Issue: 6 509-517 Turkish Journal of Agriculture and Forestry |
| ISSN: | 1303-6173 1300-011X |
| Popis: | Sugar beet (Beta vulgaris L.) is one of the most important industrial crops throughout world. With the availability of suitable genetic transformation technologies, the yield, quality, and stress tolerance of sugar beet could be improved significantly. However, low transformation efficiencies seriously limit the application of molecular technologies to the genetic improvement of sugar beet. With the aim of improving gene transfer techniques for sugar beet, the effect of different sucrose concentrations during cocultivation on the initial Agrobacterium-mediated transformation efficiencies in sugar beet was tested. To develop an efficient experimental system through which the effect of sucrose could be tested, first, a prolific regeneration system was optimized by testing the effect of different plant growth regulators on in vitro regeneration and rooting efficiencies from sugar beet cotyledonary node explants. The highest mean number of regenerated shoots per explant was obtained when the cotyledonary node explants excised from young seedlings were grown on MS medium supplemented with 1.0 mg/L 6-benzylaminopurine. Using this regeneration system, the effect of different concentrations of sucrose included in the cocultivation medium on the initial genetic transformation efficiencies observed in T0 plants was tested using an Agrobacterium tumefaciens strain carrying the pBin19/35S:GUS-INT construct. The inclusion of 4.5% sucrose in the cocultivation medium resulted in significantly higher transformation (34.09%) and expression efficiencies (22.72%), confirmed by polymerase chain reaction and β-glucuronidase assays, respectively, in regenerated T0 seedlings. If translated into stably inherited transformation efficiencies, these findings could contribute to the success of genetic transformation studies in sugar beet and other crops recalcitrant to Agrobacterium-mediated transformation. |
| Databáze: | OpenAIRE |
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