Changes in Denitrification Potentials and Riverbank Soil Bacterial Structures along Shibetsu River, Japan
Autor: | Hirosato Mogi, Misato Toda, Miyuki Oka, Toru Hamamoto, Miwako Nagane, Yoshitaka Uchida, Moe Shimotsuma, Yuto Maeda, Yui Yoshii |
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Jazyk: | angličtina |
Rok vydání: | 2018 |
Předmět: |
food.ingredient
Denitrification Article Subject Soil Science 010501 environmental sciences 01 natural sciences chemistry.chemical_compound food Nitrate Soil pH Gammaproteobacteria Aeromonadaceae Ecosystem lcsh:Agriculture (General) lcsh:Environmental sciences 0105 earth and related environmental sciences Earth-Surface Processes lcsh:GE1-350 biology Chemistry 04 agricultural and veterinary sciences biology.organism_classification lcsh:S1-972 Environmental chemistry Soil water Tolumonas 040103 agronomy & agriculture 0401 agriculture forestry and fisheries |
Zdroj: | Applied and Environmental Soil Science, Vol 2018 (2018) |
ISSN: | 1687-7667 |
DOI: | 10.1155/2018/2530946 |
Popis: | Riverbank soil ecosystems are important zones in terms of transforming inorganic nitrogen (N), particularly nitrate (NO3−-N), in soils to nitrous oxide (N2O) gases. Thus, the gasification of N in the riverbank soil ecosystems may produce a greenhouse gas, N2O, when the condition is favourable for N2O-producing microbes. One of the major N2O-producing pathways is denitrification. Thus, we investigated the denitrification potentials along Shibetsu River, Hokkaido, Japan. We sampled riverbank soils from eight sites along the Shibetsu River. Their denitrification potentials with added glucose-carbon (C) and NO3−-N varied from 4.73 to 181 μg·N·kg−1·h−1. The increase of the denitrification after the addition of C and N was negatively controlled by soil pH and positively controlled by soil NH4+-N levels. Then, we investigated the changes in 16S rRNA bacterial community structures before and after an anaerobic incubation with added C and N. We investigated the changes in bacterial community structures, aiming to identify specific microbial species related to high denitrification potentials. The genus Gammaproteobacteria AeromonadaceaeTolumonaswas markedly increased, from 0.0 ± 0.0% to 16 ± 17%, before and after the anaerobic incubation with the excess substrates, when averaged across all the sites. Although we could not find a significant interaction between the denitrification potential and the increase rate of G. AeromonadaceaeTolumonas, our study suggested that along the Shibetsu River, bacterial response to added excess substrates was similar at the genus level. Further studies are needed to investigate whether this is a universal phenomenon even in other rivers. |
Databáze: | OpenAIRE |
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