Fluorescent Arc/Arg3.1 indicator mice: a versatile tool to study brain activity changes in vitro and in vivo
| Autor: | Dietmar Kuhl, Peter H. Seeburg, Hiroshi Takuma, Pavel Osten, Jack Waters, Ryuichi Shigemoto, Naoki Takada, Alexander Kolleker, Marina Eliava, Valery Grinevich, Yugo Fukazawa |
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| Jazyk: | angličtina |
| Rok vydání: | 2009 |
| Předmět: |
Genetically modified mouse
Male Time Factors Green Fluorescent Proteins Sensory system Mice Transgenic Nerve Tissue Proteins Receptors Cell Surface Biology Fluorescence Article Amyloid beta-Protein Precursor Mice In vivo Alzheimer Disease Stress Physiological Presenilin-2 Biological neural network Presenilin-1 Animals Humans Promoter Regions Genetic Neurons Arc (protein) General Neuroscience Brain In vitro Cell biology Protease Nexins Cytoskeletal Proteins Disease Models Animal Neuroscience Immediate early gene |
| Zdroj: | Journal of Neuroscience Methods |
| Popis: | The brain-specific immediate early gene Arc/Arg3.1 is induced in response to a variety of stimuli, including sensory and behavior-linked neural activity. Here we report the generation of transgenic mice, termed TgArc/Arg3.1-d4EGFP, expressing a 4-hour half-life form of enhanced green fluorescent protein (d4EGFP) under the control of the Arc/Arg3.1 promoter. We show that d4EGFP-mediated fluorescence faithfully reports Arc/Arg3.1 induction in response to physiological, pathological and pharmacological stimuli, and that this fluorescence permits electrical recording from activated neurons in the live mouse. Moreover, the fluorescent Arc/Arg3.1 indicator revealed activity changes in circumscribed brain areas in distinct modes of stress and in a mouse model of Alzheimer’s disease. These findings identify the TgArc/Arg3.1-d4EGFP mouse as a versatile tool to monitor Arc/Arg3.1 induction in neural circuits, both in vitro and in vivo. |
| Databáze: | OpenAIRE |
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