A mixed matrix of 3-hydroxypicolinic acid and pyrazinecarboxylic acid for matrix-assisted laser desorption/ionization time-of-flight mass spectrometry of oligodeoxynucleotides
| Autor: | Huimin Deng, Lihua Zhou, Qinying Deng, Shankai Zhao |
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| Rok vydání: | 2004 |
| Předmět: |
Resolution (mass spectrometry)
Base Sequence Organic Chemistry Molecular Sequence Data Analytical chemistry Reproducibility of Results DNA 3-Hydroxypicolinic acid Mass spectrometry Pyrazinamide Analytical Chemistry Ion Solutions chemistry.chemical_compound chemistry Surface-area-to-volume ratio Oligodeoxyribonucleotides Ionization Desorption Spectrometry Mass Matrix-Assisted Laser Desorption-Ionization Acetonitrile Picolinic Acids Spectroscopy |
| Zdroj: | Rapid communications in mass spectrometry : RCM. 18(7) |
| ISSN: | 0951-4198 |
| Popis: | A mixed matrix of 3-hydroxypicolinic acid (3-HPA) and pyrazinecarboxylic acid (PCA) was used for analysis of a variety of synthetic oligodeoxynucleotides ranging in length from 8–108-mers by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. The experimental results showed that DNA segments with masses in the range from 5000 to 10 500 Da can be analyzed with high resolution (isotopic peaks resolved) when 3-HPA/PCA was prepared by mixing saturated 3-HPA solution and saturated PCA solution (in 50% of a 0.5 mol/L solution of diammonium hydrogen citrate plus 50% acetonitrile) at the volume ratio of 4:1. Each component of a mixture of d(A)1–10 gave a well-resolved peak. Moreover, when 3-HPA/PCA was used to analyze two mixtures, one containing two 23-mer DNA segments with a 9 Da difference (A and T) and the other with a 7 Da difference (AA and TG), the two 23-mer ion peaks were well separated from each other and an isotopically resolved spectrum of each component was recorded. Under the identical experimental conditions, the sample-to-sample reproducibility, resolution, signal-to-noise ratio and the tolerance to metal salts, with 3-HPA/PCA, were superior to those observed using 3-HPA alone for analysis of DNA segments. Copyright © 2004 John Wiley & Sons, Ltd. |
| Databáze: | OpenAIRE |
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