Identification of 19-hydroxy-progesterone in human placenta
| Autor: | Edelin K, F.B Field, Kyei-Aboagye K, George T. Griffing, F A Bencsath, James C. Melby, M. M. Holbrook, M. E. Ehlers |
|---|---|
| Rok vydání: | 1988 |
| Předmět: |
medicine.medical_specialty
medicine.medical_treatment Placenta Biology Mass spectrometry Biochemistry High-performance liquid chromatography Mass Spectrometry Steroid Endocrinology Pregnancy Internal medicine medicine Hydroxyprogesterones Humans Chromatography High Pressure Liquid Chromatography Human placenta Acetylation Thin-layer chromatography medicine.anatomical_structure Hydroxyprogesterone Female Chromatography Thin Layer |
| Zdroj: | Journal of steroid biochemistry. 31(4A) |
| ISSN: | 0022-4731 |
| Popis: | We have tentatively demonstrated the presence of a 19-hydroxylated C21 steroid, 19-hydroxy-progesterone, in normal human placenta. A 19-hydroxylated steroid such as 19-hydroxy-progesterone, if produced by the placenta, could serve as a precursor for such hypertensinogenic 19-nor-steroids as 19-nor-deoxycorticosterone and 19-nor-progesterone. Freshly delivered, homogenized placental tissue was extracted and subjected to thin layer chromatography. A steroid corresponding to standard 19-hydroxy-progesterone was subsequently purified in HPLC, where authentic 19-hydroxy-progesterone and the sample had the same retention time. The identity of the sample was further confirmed by repeat HPLC after acetylation and mass spectrometry. Our experiment indicates that 19-hydroxy-progesterone is present in term placental tissue, where it appears to be synthesized. |
| Databáze: | OpenAIRE |
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