Helix aspersa maxima mucus exhibits antimelanogenic and antitumoral effects against melanoma cells
| Autor: | Jed Jebali, Manel Ben Hammouda, Chedli Ellijimi, Wassim Moslah, Naziha Marrakchi, Khadija Essafi-Benkhadir, Hazem Ben Mabrouk, Najet Srairi-Abid, Maram Morjen, Houcemeddine Othman, Meriam Haoues, José Luis |
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| Přispěvatelé: | Université de Tunis El Manar (UTM), Laboratoire des Venins et Biomolécules Thérapeutiques - Laboratory of Venoms and Therapeutic Biomolecules (LR11IPT08), Institut Pasteur de Tunis, Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP), Institut de neurophysiopathologie (INP), Centre National de la Recherche Scientifique (CNRS)-Aix Marseille Université (AMU), Laboratoire d'Epidémiologie Moléculaire et de Pathologie Expérimentale Appliquée aux Maladies Infectieuses (LR11IPT04), Université de Tunis El Manar (UTM)-Institut Pasteur de Tunis, Aix Marseille Université (AMU)-Centre National de la Recherche Scientifique (CNRS) |
| Rok vydání: | 2017 |
| Předmět: |
Integrins
Carcinogenesis Cell Survival [SDV.CAN]Life Sciences [q-bio]/Cancer Apoptosis [SDV.BC]Life Sciences [q-bio]/Cellular Biology Snail Matrix Metalloproteinase Inhibitors Melanin 030207 dermatology & venereal diseases 03 medical and health sciences 0302 clinical medicine Cell Movement biology.animal Cell Line Tumor Cell Adhesion Skin cancer Animals Humans Secretion Neoplasm Invasiveness Viability assay [SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry Molecular Biology/Biochemistry [q-bio.BM] Cell adhesion Melanoma Snail slime Pharmacology Melanins biology Chemistry Helix Snails General Medicine 3. Good health Cell biology HaCaT Mucus Cell culture 030220 oncology & carcinogenesis Matrix Metalloproteinase 2 |
| Zdroj: | Biomedicine & Pharmacotherapy Biomedicine & Pharmacotherapy, 2018, 101, pp.871-880. ⟨10.1016/j.biopha.2018.03.020⟩ Biomedicine and Pharmacotherapy Biomedicine and Pharmacotherapy, 2018, 101, pp.871-880. ⟨10.1016/j.biopha.2018.03.020⟩ |
| ISSN: | 1950-6007 0753-3322 |
| Popis: | International audience; Snail secretion is currently revolutionizing the world of cosmetics and human skin care. The efficacy of snail secretion in wounds healing has been proven both in vitro and by clinical studies. However, the potential anti-tumor effect of snail secretion was poorly investigated. In this report, our in vitro study showed that Helix aspersa maxima species snail slime (SS) could not only treat melanogenesis but also endowed with anti-tumoral activity against human melanoma cells. Indeed, SS reduced melanin content and tyrosinase activity on B16F10 cells with IC50 values of 288 μg/mL and 286 μg/mL, respectively, without altering cell viability. This effect was also observed, at a lesser extent, on human melanoma IGR-39 and SK-MEL-28 cell lines. On another hand, SS specifically inhibited the viability of IGR-39 and SK-MEL-28 cells associated to an apoptotic effect highlighted by PARP cleavage. It is worth to note that SS did not affect the viability of B16F10 cells and non tumorigenic HaCaT cells. Interestingly, this extract was found to inhibit migration and invasion of both human melanoma cells through reducing the expression of Matrix metalloproteinase MMP2. Snail slime also exerted a high inhibitory effect on IGR-39 cell adhesion through blocking the function of α2β1 (45%), αvβ3 (38%) integrins and by reducing the expression levels of αv and β1 integrins. The presented results shed light on the potential anti-melanoma effect of SS and support its use against skin diseases. |
| Databáze: | OpenAIRE |
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