16S rRNA gene taxonomic profiling of endophytic bacteria associated with phylaenopsis roots
| Autor: | Mahesh Mohan, D. Girija, P.K. Rajeevan, Swathi Balakrishnan, P.S. Panchami |
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| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Operational taxonomic unit biology Phylum 030106 microbiology Short Communications Plant culture Plant Science Horticulture Ribosomal RNA biology.organism_classification 16S ribosomal RNA Actinobacteria SB1-1110 03 medical and health sciences Metagenomics Botany Endophyte orchid diversity Rubrobacter Proteobacteria Ecology Evolution Behavior and Systematics |
| Zdroj: | Journal of Horticultural Sciences Journal of Horticultural Sciences, Vol 13, Iss 1, Pp 103-107 (2018) |
| ISSN: | 2582-4899 0973-354X |
| Popis: | Orchids are one of the main groups of ornamental plants commercially exploited. In the present study, we analyzed the diversity of bacterial community in Phalaenopsis root using metagenomic approach. The diversity of bacterial taxonomic category was assessed at different Operational Taxonomic Unit (OTU) levels using Ribosomal Database Project (RDP) pipeline and MG-RAST. At phylum level, Proteobacteria (61.34%) was the most dominant group followed by unclassified derived from bacteria (24.74%) and Actinobacteria (12.52%). Genus level analysis revealed the abundance of Rubrobacter, Pseudomonas and Acinetobacter. The study revealed that of the total species detected 50.83 per cent were unclassified, stressing the importance of metagenomics to assess the diversity of endophytes associated with orchid roots. |
| Databáze: | OpenAIRE |
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