16S rRNA gene taxonomic profiling of endophytic bacteria associated with phylaenopsis roots

Autor: Mahesh Mohan, D. Girija, P.K. Rajeevan, Swathi Balakrishnan, P.S. Panchami
Jazyk: angličtina
Rok vydání: 2018
Předmět:
Zdroj: Journal of Horticultural Sciences
Journal of Horticultural Sciences, Vol 13, Iss 1, Pp 103-107 (2018)
ISSN: 2582-4899
0973-354X
Popis: Orchids are one of the main groups of ornamental plants commercially exploited. In the present study, we analyzed the diversity of bacterial community in Phalaenopsis root using metagenomic approach. The diversity of bacterial taxonomic category was assessed at different Operational Taxonomic Unit (OTU) levels using Ribosomal Database Project (RDP) pipeline and MG-RAST. At phylum level, Proteobacteria (61.34%) was the most dominant group followed by unclassified derived from bacteria (24.74%) and Actinobacteria (12.52%). Genus level analysis revealed the abundance of Rubrobacter, Pseudomonas and Acinetobacter. The study revealed that of the total species detected 50.83 per cent were unclassified, stressing the importance of metagenomics to assess the diversity of endophytes associated with orchid roots.
Databáze: OpenAIRE