Dopamine down-regulates activity of alkaline phosphatase in Drosophila: The role of D2-like receptors
Autor: | E. V. Bogomolova, A. A. Alekseev, I. Yu. Rauschenbach, N. V. Adonyeva, N. V. Faddeeva, N. E. Gruntenko |
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Rok vydání: | 2010 |
Předmět: |
musculoskeletal diseases
Agonist medicine.medical_specialty Hot Temperature Physiology medicine.drug_class Dopamine Gene Expression Regulation Enzymologic Species Specificity stomatognathic system Stress Physiological Internal medicine medicine Animals Receptor Bromocriptine Chromatography High Pressure Liquid biology Receptors Dopamine D2 musculoskeletal neural and ocular physiology fungi Alkaline Phosphatase musculoskeletal system biology.organism_classification Enzyme assay Dihydroxyphenylalanine Drosophila virilis Endocrinology D2-like receptor Insect Science biology.protein Alkaline phosphatase Drosophila Female RNA Interference Spectrophotometry Ultraviolet medicine.drug |
Zdroj: | Journal of Insect Physiology. 56:1155-1159 |
ISSN: | 0022-1910 |
Popis: | The effect of a rise in dopamine (DA) level as a result of a mutation, stress or pharmacological treatment on the activity of the enzyme of its synthesis, alkaline phosphatase (ALP) in females of Drosophila virilis and Drosophila melanogaster has been studied. It has been found that regardless of its nature, a rise in DA level has a negative effect on ALP activity, which indicates that DA down-regulates activity of the enzyme. The effects of bromocriptine (an agonist of Drosophila dopamine 2-like receptor (DD2R)) on ALP activity have been studied. ALP activity was found to drop in response to bromocriptine in flies. Conversely ALP activity was increased in flies with reduced DD2R expression (i.e. Actin5C-Gal4>UAS-ds-DD2R RNA-interference flies) vs. corresponding controls (i.e. Actin5C-Gal4>w1118 flies). Bromocriptine treatment of RNAi flies rescues ALP activity to the level typical of Actin5C-Gal4>w1118 flies. A change in DD2R number or availability was found not to prevent the response of ALP to heat stress, but to change the intensity of its response to the stress exposure. The role of D2-like receptors in down-regulation of ALP activity by DA and in ALP response to stressor in Drosophila is discussed. |
Databáze: | OpenAIRE |
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