High-Efficiency Rescue of Equine Infectious Anemia Virus from a CMV-Driven Infectious Clone
| Autor: | Yue-Zhi Lin, Xiaojun Wang, Mingxin Song, Ting Qi, Cheng Du, Bowen Bai, Xue-Feng Wang |
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| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
medicine.medical_specialty Letter Transcription Genetic animal diseases viruses 030106 microbiology Immunology Clone (cell biology) Cytomegalovirus Biology Virus Cell Line Equine infectious anemia 03 medical and health sciences Medical microbiology Virology medicine Animals Humans Horses Promoter Regions Genetic Virus Release virus diseases Viral Vaccines biochemical phenomena metabolism and nutrition biology.organism_classification Blot Equine Infectious Anemia HEK293 Cells 030104 developmental biology Viral replication Cell culture Molecular Medicine Infectious Anemia Virus Equine |
| Zdroj: | Virol Sin |
| ISSN: | 1995-820X 1674-0769 |
| DOI: | 10.1007/s12250-019-00153-w |
| Popis: | In this study, the strong promoter of CMV was introduced in place of the 5' LTR U3 of EIAV to improve the expression level of the molecular clone and to promote the rescue efficiency of the virus. We demonstrated that the EIAV infectious clone pCMV3-8 have a strong capacity to rescue EIAV by viral RT activity assay, Western blotting and TEM. Taken together, these results indicate that pCMV3-8 could be used for genetic manipulation of EIAV and can be applied to the researches on the mechanism of viral replication and attenuated EIAV vaccine. |
| Databáze: | OpenAIRE |
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