Defective expression of IL-2 receptors on peripheral blood lymphocytes from patients with cluster headache
| Autor: | Francesco Di Sabato, G. Stirparo, Mario Giacovazzo, L. Stefano, Cristina Rinaldi-Garaci, R Martelletti |
|---|---|
| Rok vydání: | 1990 |
| Předmět: |
Male
Receptor expression CD3 T-Lymphocytes Fc receptor Transferrin receptor Cluster Headache CD16 Medicine Humans IL-2 receptor Receptor biology business.industry Becton dickinson Antibodies Monoclonal Receptors Interleukin-2 Molecular biology Vascular Headaches Neurology Immunology Acute Disease biology.protein Female Neurology (clinical) business |
| Zdroj: | Headache. 30(4) |
| ISSN: | 0017-8748 |
| Popis: | SYNOPSIS In a previous study we demonstrated that cluster headache (CH) patients present an increased Natural Cytotoxic response after incubation of their peripheral blood lymphocytes (PBL) with Interleukin-2 (IL-2). This phenomenon led to an investigation of the phenotypic expression of PBL before and after IL-2 incubation, and of the IL-2 lymphocyte receptor. IL-2 receptor is expressed on T-lymphocytes activated with an high-affinity binding site. The analysis of the function of human IL-2 receptor was facilitated by the production of a specific monoclonal antibody (MAb). This MAb identifies the IL-2 receptors by blocking the binding of radiolabelled IL-2 to T-cells. In addition, we studied the expression of Leu-4, specific for T-cells; of Leu-11b, specific for FC receptor on NK cells; and the Transferrin Receptor, specific for lymphoblasts and monocytes. Twenty-three episodic CH patients were selected for this study. Ten sex and age-matched healthy volunteers were used as the control group. We evaluated the PBL phenotypic expression of cells subsets before incubation with IL-2 (1,000 I.U./ml) and after 72 hours. The following Becton Dickinson MAbs have been used: anti-Leu-4 (CD3), anti-lL-2 receptors (CD25), anti-Transferrin receptor (TFR) and anti-Leu-11b (CD16). Indirect fluorescence with a Becton Dickinson FACS-420 flow cytometer was used to analyze the cells. The results showed no significant variations in Leu-4, Leu-11b and Transferrin receptor in PBL of CH patients when compared to those of control subjects, both before and after incubation with IL-2, whereas the percentage of PBL expressing receptors for IL-2 was markedly reduced in CH patients as compared to normal individuals after incubation with IL-2. This defective expression of IL-2 receptors seems to be independent of the cluster period. These data suggest that the failure of IL-2 receptor expression in CH can be inserted into a complex network of abnormalities of the immunoneuromodulating system in this disease. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text