Identification of two novel foot-and-mouth disease virus cytotoxic T lymphocyte epitopes that can bind six SLA-I proteins
| Autor: | Ping Jiang, Qiang Zhang, Yong Han, Zong-hui Zhang, Zi-bin Li, Xiao-xin Zhai, Hua Gao, Jie Yang, Feng-Shan Gao |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Male Antigenicity Swine Epitopes T-Lymphocyte chemical and pharmacologic phenomena Biology Major histocompatibility complex Epitope 03 medical and health sciences 0302 clinical medicine Species Specificity Genetics Escherichia coli Cytotoxic T cell Animals ELISPOT hemic and immune systems General Medicine biology.organism_classification Virology Antibodies Neutralizing CTL 030104 developmental biology Foot-and-Mouth Disease Virus Foot-and-Mouth Disease biology.protein Foot-and-mouth disease virus Antibody 030215 immunology Protein Binding T-Lymphocytes Cytotoxic |
| Zdroj: | Gene. 653 |
| ISSN: | 1879-0038 |
| Popis: | Currently available vaccines from inactivated foot-and-mouth disease virus (FMDV) only protect animals by inducing neutralizing antibodies. A vaccine that contains cytotoxic T lymphocytes (CTL) epitopes to induce strong CTL responses might protect animals more effectively. Herein, we used swine leukocyte antigen class I (SLAI) proteins derived from six different strains of domestic pigs to screen and identify shared FMDV CTL epitopes. Four potential FMDV CTL epitopes (Q01, Q02, AS3, and QA4) were confirmed by mass spectrometry. We also determined the antigenicity of these epitopes to elicit cell-mediated immunoresponse by the ELISPOT and CTL assays. Among the four peptides, Q01 and QA4 were found to bind all six SLA-I proteins with strong affinity and elicit significant activity of CTL (P 0.01). We conclude that Q01 and QA4 peptides are novel shared epitopes that can be recognized by all six SLA-I molecules on representative CTLs. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect