Identification of a major-effect QTL associated with pre-harvest sprouting in cucumber (Cucumis sativus L.) using the QTL-seq method
Autor: | Qiang Deng, Ming ming Cao, Hui zhe Wang, Shu ju Li, Rui huan Yang |
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Jazyk: | angličtina |
Rok vydání: | 2021 |
Předmět: |
0106 biological sciences
Candidate gene lcsh:QH426-470 lcsh:Biotechnology Population Quantitative Trait Loci Quantitative trait locus 01 natural sciences Polymorphism Single Nucleotide 03 medical and health sciences lcsh:TP248.13-248.65 Genetics qPHS4.1 education Indel 030304 developmental biology Pre-harvest sprouting 0303 health sciences education.field_of_study biology Cucumber Chromosome Chromosome Mapping food and beverages biology.organism_classification lcsh:Genetics Plant Breeding Chromosome 4 QTL-seq DNA microarray Cucumis sativus Cucumis 010606 plant biology & botany Biotechnology Research Article |
Zdroj: | BMC Genomics BMC Genomics, Vol 22, Iss 1, Pp 1-11 (2021) |
ISSN: | 1471-2164 |
Popis: | Background Cucumber (Cucumis sativus L.) is cultivated worldwide, and it is essential to produce enough high-quality seeds to meet demand. Pre-harvest sprouting (PHS) in cucumber is a critical problem and causes serious damage to seed production and quality. Nevertheless, the genetic basis and molecular mechanisms underlying cucumber PHS remain unclear. QTL-seq is an efficient approach for rapid quantitative trait loci (QTL) identification that simultaneously takes advantage of bulked-segregant analysis (BSA) and whole-genome resequencing. In the present research, QTL-seq analysis was performed to identify QTLs associated with PHS in cucumber using an F2 segregating population. Results Two QTLs that spanned 7.3 Mb on Chromosome 4 and 0.15 Mb on Chromosome 5 were identified by QTL-seq and named qPHS4.1 and qPHS5.1, respectively. Subsequently, SNP and InDel markers selected from the candidate regions were used to refine the intervals using the extended F2 populations grown in the 2016 and 2017 seasons. Finally, qPHS4.1 was narrowed to 0.53 Mb on chromosome 4 flanked by the markers SNP-16 and SNP-24 and was found to explain 19–22% of the phenotypic variation in cucumber PHS. These results reveal that qPHS4.1 is a major-effect QTL associated with PHS in cucumber. Based on gene annotations and qRT-PCR expression analyses, Csa4G622760 and Csa4G622800 were proposed as the candidate genes. Conclusions These results provide novel insights into the genetic mechanism controlling PHS in cucumber and highlight the potential for marker-assisted selection of PHS resistance breeding. |
Databáze: | OpenAIRE |
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