Impaired Collateral Flow in Pial Arterioles of Aged Rats During Ischemic Stroke
Autor: | Yonglie Ma, Junqiang Ma, Ian R. Winship, Ashfaq Shuaib |
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Rok vydání: | 2019 |
Předmět: |
Male
0301 basic medicine Aging Middle Cerebral Artery medicine.medical_specialty Anterior Cerebral Artery Ischemia Cerebral circulation Brain Ischemia Rats Sprague-Dawley 03 medical and health sciences 0302 clinical medicine Arteriole medicine.artery Internal medicine Anterior cerebral artery medicine Animals Stroke Ischemic Stroke Cerebral Cortex business.industry General Neuroscience Optical Imaging Collaterals Blood flow medicine.disease Collateral circulation Arterioles 030104 developmental biology Middle cerebral artery Cardiology Original Article Neurology (clinical) Cardiology and Cardiovascular Medicine business 030217 neurology & neurosurgery |
Zdroj: | Translational Stroke Research |
ISSN: | 1868-601X 1868-4483 |
Popis: | Cerebral collateral circulation and age are critical factors in determining outcome from acute ischemic stroke. Aging may lead to rarefaction of cerebral collaterals, and thereby accelerate ischemic injury by reducing penumbral blood flow. Dynamic changes in pial collaterals after onset of cerebral ischemia may vary with age but have not been extensively studied. Here, laser speckle contrast imaging (LSCI) and two-photon laser scanning microscopy (TPLSM) were combined to monitor cerebral pial collaterals between the anterior cerebral artery (ACA) and the middle cerebral artery (MCA) in young adult and aged male Sprague Dawley rats during distal middle cerebral artery occlusion (dMCAo). Histological analysis showed that aged rats had significantly greater volumes of ischemic damage than young rats. LSCI showed that cerebral collateral perfusion declined over time after stroke in aged and young rats, and that this decline was significantly greater in aged rats. TPLSM demonstrated that pial arterioles narrowed faster after dMCAo in aged rats compared to young adult rats. Notably, while arteriole vessel narrowing was comparable 4.5 h after ischemic onset in aged and young adult rats, red blood cell velocity was stable in young adults but declined over time in aged rats. Overall, red blood cell flux through pial arterioles was significantly reduced at all time-points after 90 min post-dMCAo in aged rats relative to young adult rats. Thus, collateral failure is more severe in aged rats with significantly impaired pial collateral dynamics (reduced diameter, red blood cell velocity, and red blood cell flux) relative to young adult rats. |
Databáze: | OpenAIRE |
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