Comparison of six different culture media for isolation of Treponema hyodysenteriae

Autor:	Serge Messier, M Achacha
Rok vydání:	1992
Předmět:	Swine Diseases Microbiology (medical) Fecal flora Treponema Treponemal Infections biology Swine Inoculation biology.organism_classification Isolation (microbiology) Culture Media Dysentery Microbiology Feces Animals Food science Refrigeration temperature Bacteria Research Article
Zdroj:	Journal of Clinical Microbiology. 30:249-251
ISSN:	1098-660X 0095-1137
DOI:	10.1128/jcm.30.1.249-251.1992
Popis:	Fecal material from pigs experimentally infected with Treponema hyodysenteriae was collected and inoculated on six different selective media to compare their abilities for recovery of T. hyodysenteriae. Additionally, various types of samples were compared to find the most appropriate for submitting material to laboratories. Isolation rates for T. hyodysenteriae were 89.7% on BJ medium, 88.3% on TSA-BJ medium, 76.6% on SVC medium, 76.6% on BA2-BJ medium, 75.2% on BA2-SVC medium, and 52.4% on TSA-S400 medium. BJ medium was the most useful, yielding good growth of T. hyodysenteriae and inhibition of the normal fecal flora. Also, undiluted fecal material kept at refrigeration temperature allowed better recovery of T. hyodysenteriae during storage than swabs.
Databáze:	OpenAIRE
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