Loranthus micranthusnanoparticles abates streptozotocin‐instigated testicular dysfunction in Wistar rats: Involvement of glucose metabolism enzymes, oxido‐inflammatory stress, steroidogenic enzymes/protein and Nrf2 pathway
| Autor: | Sharoon Griffin, Olasehinde Olusanya, Cornelia M. Keck, Sharon O. Osawe, Richard C. Ehiri, Azubuike P. Ebokaiwe |
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| Rok vydání: | 2020 |
| Předmět: |
Blood Glucose
Male medicine.medical_specialty endocrine system diseases NF-E2-Related Factor 2 Urology medicine.medical_treatment Intraperitoneal injection 030232 urology & nephrology Blood sugar Carbohydrate metabolism Loranthaceae Streptozocin Diabetes Mellitus Experimental Lipid peroxidation 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Endocrinology Internal medicine Diabetes mellitus Testis medicine Animals Humans Rats Wistar 030219 obstetrics & reproductive medicine business.industry Fructose General Medicine Streptozotocin medicine.disease Rats Metformin Oxidative Stress Glucose chemistry Nanoparticles business medicine.drug |
| Zdroj: | Andrologia. 52 |
| ISSN: | 1439-0272 0303-4569 |
| Popis: | Loranthus micranthus (African mistletoe)-Loranthaceae family, is used in Nigerian traditional medicine for treating male infertility and lowering diabetic blood sugar levels. We investigated possible mechanism(s) involved in mitigation of L. micranthus leaves nanoparticles (LMLNPs) on streptozotocin (STZ)-induced testicular alterations. Type two diabetes mellitus (T2DM) was induced in male rats following 2 weeks feeding with fructose and single intraperitoneal injection of STZ. Control (nondiabetic) and (diabetic) rats received buffer only. Diabetic rats were treated with metformin or LMLNPs (two different doses) for 28 days. Hormonal profile, oxido-inflammatory stress parameters, glucose metabolism and steroidogenic enzymes/regulatory protein (StAR) and Nuclear factor erythroid 2-related factor 2 (Nrf2) protein in testes and sperm parameters were evaluated. Metformin and LMLNPs treatment significantly reduced blood glucose level in diabetic rats. Furthermore, LMLNPs enhanced glucose metabolism and testicular steroidogenic enzymes/protein, increased reproductive hormone levels and sperm functional parameters in diabetic rats. Additionally, LMLNPs suppressed testicular oxido-inflammatory stress biomarkers and inhibited lipid peroxidation in diabetic rats while augmenting Nrf2 pathway. Conclusively, LMLNPs potently reversed adverse effects of T2DM testicular dysfunction of rats. Use of LMLNPs in abating diabetic consequences proves an acceptable alternative to traditional crude extract preparations, as a result of better packaging and preservation. |
| Databáze: | OpenAIRE |
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