Galactomannan hydrolysis and mannose metabolism inCellvibrio mixtus
| Autor: | Maria S.J. Centeno, Louise E. Tailford, Fernando M. V. Dias, Karen E. Nelson, R.M. Caldeira, Emmanuel F. Mongodin, Carl Morland, Arun Goyal, Carlos M. G. A. Fontes, Catarina I. P. D. Guerreiro, Harry J. Gilbert, L M A Ferreira, José A.M. Prates |
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| Rok vydání: | 2006 |
| Předmět: |
Aerobic bacteria
Cellvibrio Molecular Sequence Data Racemases and Epimerases Mannose Polysaccharide Microbiology Mannans Hydrolysis Galactomannan chemistry.chemical_compound Bacterial Proteins Escherichia coli Genetics Glycoside hydrolase Amino Acid Sequence Cloning Molecular Molecular Biology Phylogeny chemistry.chemical_classification biology Galactose biology.organism_classification chemistry Biochemistry Multigene Family alpha-Galactosidase Sequence Alignment |
| Zdroj: | FEMS Microbiology Letters. 261:123-132 |
| ISSN: | 1574-6968 0378-1097 |
| DOI: | 10.1111/j.1574-6968.2006.00342.x |
| Popis: | Galactomannan hydrolysis results from the concerted action of microbial endo-mannanases, manosidases and alpha-galactosidases and is a mechanism of intrinsic biological importance. Here we report the identification of a gene cluster in the aerobic soil bacterium Cellvibrio mixtus encoding enzymes involved in the degradation of this polymeric substrate. The family 27 alpha-galactosidase, termed CmAga27A, preferentially hydrolyse galactose containing polysaccharides. In addition, we have characterized an enzyme with epimerase activity, which might be responsible for the conversion of mannose into glucose. The role of the identified enzymes in the hydrolysis of galactomannan by aerobic bacteria is discussed. |
| Databáze: | OpenAIRE |
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