New staining methods for sperm evaluation estimated by microscopy and flow cytometry

Autor: J.Cl. Nicolle, Eric Palmer, Y. Levern, Dominique Kerboeuf, Marianne Vidament, Michèle Magistrini, E. Guitton
Přispěvatelé: Unité de recherche Physiologie de la reproduction des mammifères domestiques, Nouzilly, Institut National de la Recherche Agronomique (INRA), Station de Pathologie aviaire et parasitologie [Nouzilly] (PAP), ProdInra, Migration
Jazyk: angličtina
Rok vydání: 1997
Předmět:
Pathology
medicine.medical_specialty
040301 veterinary sciences
[SDV]Life Sciences [q-bio]
Large population
Semen
[INFO] Computer Science [cs]
Biology
Flow cytometry
0403 veterinary science
Andrology
03 medical and health sciences
0302 clinical medicine
Food Animals
Capacitation
Microscopy
medicine
Fluorescence microscope
[INFO]Computer Science [cs]
Small Animals
ComputingMilieux_MISCELLANEOUS
030219 obstetrics & reproductive medicine
[SDV.BA.MVSA]Life Sciences [q-bio]/Animal biology/Veterinary medicine and animal Health
medicine.diagnostic_test
Equine
[SDV.BA.MVSA] Life Sciences [q-bio]/Animal biology/Veterinary medicine and animal Health
04 agricultural and veterinary sciences
Sperm
Staining
[SDV] Life Sciences [q-bio]
Animal Science and Zoology
EVALUATION GENETIQUE DES PERES
MICROSCOPIE EN FLUORESCENCE
Zdroj: Theriogenology
Theriogenology, Elsevier, 1997, 48, pp.1229-1235
Stallion semen production evaluation and preservation
Stallion semen production evaluation and preservation, Mar 1996, Amersfoot, Netherlands
ISSN: 0093-691X
Popis: New staining methods and automated instruments are now available to evaluate the sperm cell in vitro. Individual compartments of the sperm cell, such as the nucleus and the plasma and acrosomal membranes, may be investigated, as well as the cell function as shown by mitochondria activity and capacitation. Various probes are used and they can be analyzed by direct light or fluorescent microscopy or by flow cytometry. The automated instruments allow objective and accurate analysis and quantification as well as the ability to evaluate large population of cells in a shorter time, thus providing accurate evaluation of sperm quality. However, before these test can be recommended for routine clinical and investigational use, in the stallion, they need to be confirmed on a larger number of stallions and their correlation with traditional semen parameters and with stallion fertility has to be demonstrated.
Databáze: OpenAIRE
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