| Autor: |
Miao Zhang, Ning Wang, Xiang-Shen Guo, Lin-Lin Wang, Peng-Fei Wang, Zhi-Peng Cao, Fu-Yuan Zhang, Zi-Wei Wang, Da-Wei Guan, Rui Zhao |
| Rok vydání: |
2022 |
| Předmět: |
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| Zdroj: |
International journal of legal medicine. |
| ISSN: |
1437-1596 |
| Popis: |
Post-mortem diagnosis of fatal hypothermia (FHT) is challenging in forensic practice because traditional morphological and biochemical methods lack specificity. Recent studies have reported that brown adipose tissue (BAT) is activated during cold-induced non-shivering thermogenesis in mammals, but BAT has not been used to diagnose FHT. The aim of this study was to identify novel biomarkers in BAT for FHT based on morphological changes and differential protein expression. Two FHT animal models were created by exposing mice to 4 or -20 °C at 50% humidity. Morphologically, the unilocular lipid droplet content was significantly increased in BAT of FHT model mice compared with that of control mice. Proteomics analysis revealed a total of 283 and 266 differentially expressed proteins (DEPs) between the 4 or -20 °C FHT subgroups and control group, respectively. In addition, 140 proteins were shared between the FHT subgroups. GO and KEGG analyses revealed that the shared DEPs were mainly enriched in pathways associated with metabolism, oxidative phosphorylation, and thermogenesis. Further screening (|log |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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