Fluorescent Proteins Expressed in Mouse Transgenic Lines Mark Subsets of Glia, Neurons, Macrophages, and Dendritic Cells for Vital Examination
| Autor: | Virginia L. Scofield, Le Tian, Paul A. Krieg, Shan Maika, Alexander Marks, Michelle Mikesh, Craig S. Newman, Yi Zuo, Jane L. Lubischer, Wesley J. Thompson, Hyuno Kang |
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| Rok vydání: | 2004 |
| Předmět: |
Recombinant Fusion Proteins
Transgene Green Fluorescent Proteins Development/Plasticity/Repair Neuromuscular Junction Schwann cell Mice Transgenic S100 Calcium Binding Protein beta Subunit Biology Cell Line Green fluorescent protein Mice Lens Crystalline Adipocytes medicine Animals Humans Receptors Cholinergic Nerve Growth Factors Transgenes Neurons Microglia Macrophages General Neuroscience S100 Proteins Dendritic Cells Motor neuron Cell biology Mice Inbred C57BL Luminescent Proteins medicine.anatomical_structure Microscopy Fluorescence nervous system Cell culture Langerhans Cells Neuroglia Schwann Cells Neuroscience Astrocyte |
| Zdroj: | The Journal of Neuroscience. 24:10999-11009 |
| ISSN: | 1529-2401 0270-6474 |
| DOI: | 10.1523/jneurosci.3934-04.2004 |
| Popis: | To enable vital observation of glia at the neuromuscular junction, transgenic mice were generated that express proteins of the green fluorescent protein family under control of transcriptional regulatory sequences of the human S100B gene. Terminal Schwann cells were imaged repetitively in living animals of one of the transgenic lines to show that, except for extension and retraction of short processes, the glial coverings of the adult neuromuscular synapse are stable. In other lines, subsets of Schwann cells were labeled. The distribution of label suggests that Schwann cells at individual synapses are clonally related, a finding with implications for how these cells might be sorted during postnatal development. Other labeling patterns, some present in unique lines, included astrocytes, microglia, and subsets of cerebellar Bergmann glia, spinal motor neurons, macrophages, and dendritic cells. We show that lines with labeled macrophages can be used to follow the accumulation of these cells at sites of injury. |
| Databáze: | OpenAIRE |
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