A serine protease from the midgut of the bumblebee, Bombus ignitus (Hymenoptera: Apidae): cDNA cloning, gene structure, expression and enzyme activity
| Autor: | Hyung Joo Yoon, Sang Beom Lee, Young Moo Choo, Byung Rae Jin, Hung Dae Sohn, Kwang Sik Lee, Ji Hyeon Kim |
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| Rok vydání: | 2007 |
| Předmět: |
Serine protease
TMPRSS6 Bombus ignitus Protease baculovirus expression vector serine protease medicine.medical_treatment bumblebee gene structure Biology Molecular biology apidae enzyme genomic DNA QL1-991 Insect Science Complementary DNA cdna cloning expression medicine biology.protein bombus ignitus Zoology Gene Southern blot |
| Zdroj: | European Journal of Entomology, Vol 104, Iss 1, Pp 1-7 (2007) |
| ISSN: | 1802-8829 1210-5759 |
| Popis: | A serine protease gene was cloned from the bumblebee, Bombus ignitus. The B. ignitus serine protease (BiSP) gene spans 1702 bp and consists of four introns and five exons coding for 250 amino acid residues. Southern blot analysis of genomic DNA sug- gested that BiSP gene is a single copy gene. The cDNA encoding BiSP was expressed as a 28-kDa polypeptide in baculovirus- infected insect cells and the recombinant BiSP showed activity in a protease enzyme assay. BiSP was specifically expressed in the midgut of B. ignitus queens, males, and workers, suggesting that the BiSP is a gut enzyme involved in the digestion of dietary pro- teins. |
| Databáze: | OpenAIRE |
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