Substantial production of ghrelin by a human medullary thyroid carcinoma cell line
| Autor: | Naotetsu Kanamoto, Yuji Hataya, Kazuwa Nakao, Akira Shimatsu, Kenji Moriyama, Misa Saijo, Kazuhiko Takaya, Hiroshi Hosoda, Kenji Kangawa, Takashi Akamizu, Hiroyuki Ariyasu, Masayasu Kojima, Kiminori Hosoda |
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| Rok vydání: | 2001 |
| Předmět: |
endocrine system
medicine.medical_specialty endocrine system diseases Parafollicular cell Endocrinology Diabetes and Metabolism Peptide Hormones Clinical Biochemistry Radioimmunoassay Thyroid Gland Biology Biochemistry Follicular cell Thyroid carcinoma Endocrinology Internal medicine medicine Carcinoma Tumor Cells Cultured Humans RNA Messenger Thyroid Neoplasms Protein Precursors digestive oral and skin physiology Biochemistry (medical) Thyroid medicine.disease Immunohistochemistry Ghrelin medicine.anatomical_structure Medullary carcinoma Cell culture Carcinoma Medullary Peptides hormones hormone substitutes and hormone antagonists |
| Zdroj: | The Journal of clinical endocrinology and metabolism. 86(10) |
| ISSN: | 0021-972X |
| Popis: | Ghrelin, an endogenous ligand for the GH secretagogue receptor, is a novel acylated peptide produced in the gastrointestinal endocrine cells as well as neuroendocrine cells in the hypothalamus. The Ser(3) residue of ghrelin is modified by n-octanoic acid, a modification necessary for hormonal activity. Human medullary thyroid carcinoma is known to produce a variety of gastrointestinal and neuroendocrine peptides. In the present study we investigated ghrelin production in the thyroid gland, especially in human medullary thyroid carcinoma. PCR amplification demonstrated prepro-ghrelin gene transcripts in normal human thyroid tissue and two medullary thyroid carcinoma cell lines (human TT cells and rat 6-23 cells), but not in a rat thyroid follicular cell line. TT cells showed the expression of prepro-ghrelin mRNA of about 0.6 kb by Northern blot analysis. Furthermore, production of ghrelin in TT cells was demonstrated by RIA and immunocytochemistry. Accumulation of des-n-octanoyl ghrelin in the cultured medium of the cells was confirmed. Finally, human medullary thyroid carcinoma surgical specimens showed significantly higher des-n-octanoyl ghrelin contents than normal thyroid tissues. In conclusion, we revealed that ghrelin was produced by the human thyroid parafollicular carcinoma cell line, TT cells. These findings suggest that ghrelin is produced in the thyroid C cells as well as in medullary thyroid carcinoma and may provide opportunities to investigate its physiological role in the thyroid gland. |
| Databáze: | OpenAIRE |
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